The fluorescently labeled antibodies used for flow cytometry were purchased from the following companies, and the company protocols were followed in all applications: BioLegend (San Diego, CA, USA): anti-CD3-FITC (Cat. # 100306), anti-CD4-PerCP/Cy5.5 (Cat. # 100540), anti-B220-PerCP/Cy5.5 (Cat. # 103236), anti-CD3-PerCP/Cy5.5 (Cat. # 100328), anti-CD8-PerCP/Cy5.5 (Cat. # 100734), anti-CD8-APC (Cat. # 100712), and anti-CD279-APC (Cat. # 135209); eBioscience (San Diego, CA, USA): anti-DX5-FITC (Cat. # 11-5971-85), anti-CD274-PE (Cat. # 551892), and anti-F4/80-APC (Cat. # 17-4801-80); and BD Biosciences (San Jose, CA, USA): anti-CD 279-PE (Cat. # 551892).
Anti dx5 fitc
Manufactured by Thermo Fisher Scientific
Sourced in United States
Anti-DX5-FITC is a fluorescently labeled antibody that binds to the DX5 antigen, which is expressed on natural killer (NK) cells. It is used for the identification and quantification of NK cells in flow cytometry analysis.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd3 fitc, by BioLegend (1 mentions)
Anti cd4 percp cy5, by BioLegend (1 mentions)
Anti b220 percp cy5, by BioLegend (1 mentions)
Anti cd274 pe, by BD (1 mentions)
Anti f4 80 apc, by BD (1 mentions)
Anti cd8 apc, by Thermo Fisher Scientific (1 mentions)
Anti cd3e percp, by Thermo Fisher Scientific (1 mentions)
Facscalibur flow cytometer, by BD (1 mentions)
Anti cd8 pe, by Thermo Fisher Scientific (1 mentions)
40 μm cell strainer, by BD (1 mentions)
2 protocols using anti dx5 fitc
1
Fluorescent Antibody Flow Cytometry
2
Cytotoxic T Cell Profiling in Viral Infection
Check if the same lab product or an alternative is used in the 5 most similar protocols
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DPLN and spleens from PBS-inoculated or ECTV-infected BALB/c mice were collected at 2 and 7 dpi, respectively, in RPMI supplemented with 10% FCS. Cell suspensions were obtained by homogenization of the organs through 40 μm cell strainers (BD Bioscience). Red blood cells were lysed by hypoosmotic shock in milli-Q water and white cells were washed twice in PBS and counted manually in a haemocytometer. DPLN cells were stained with anti-DX5-FITC (eBioscience), anti-CD3e-PerCP (eBioscience) and anti-GzB-APC (R&D Biosystems). Splenocytes were stained with anti-CD3e-PerCP, anti-CD8-PE (eBioscience) and anti-GzB-APC. In parallel, cell suspensions were also stained with the appropriate isotype control antibodies. 100,000 cells were analyzed in a FACS Calibur flow cytometer (Becton Dickinson). Events were gated according to a forward and side scatter pattern compatible with healthy lymphocytes (Supplementary Fig. 3 ). Results were analyzed with FlowJo software (FlowJo LLC).
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