4 dimethylaminopyridine dmap

Manufactured by Maclin Biochemical Technology

Sourced in China

4-Dimethylaminopyridine (DMAP) is a chemical compound that functions as a nucleophilic catalyst. It is commonly used in organic synthesis reactions to facilitate various transformations.

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6 protocols using 4 dimethylaminopyridine dmap

Chitosan-PVA Hydrogel for Wound Healing

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Poly (vinyl alcohol) 124 (PVA-124, Mw~105 kDa, degree of hydrolysis 98.0–99.8 mol%), barium chloride (BaCl₂), and ammonium sulfate ((NH₄)₂SO₄) were obtained from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Thrombin and chitosan (CS, Mw = 110–150 kDa, 93% degree of deacetylation) were provided by Sigma-Aldrich (Beijing, China). Glutaraldehyde (GA, 50 wt%) and 4-dimethylaminopyridine (DMAP) were received from Macklin Biochemical Co., Ltd. (Shanghai, China). Succinic anhydrate, 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid (HEPES), 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC), and N-hydroxysuccinimide (NHS) were obtained from Aladdin Reagent Co., Ltd. (Shanghai, China). Fetal bovine serum (FBS), DMEM medium, and antibiotics (penicillin: 100 IU/mL + streptomycin: 100 mg/mL) were provided by Gibco Life Technology Co., Ltd. (Grand Island, USA). CCK-8 assay kit was produced by Dojindo Laboratories (Kumamoto, Japan).
In vivo animal tests were performed on New Zealand white rabbits supplied by the Experimental Animal Center in our institution, and the experiments were complied with the guidelines established by the Institutional Animal Care and Use Committee.

Li X., Ji X., Chen K., Ullah M.W., Li B., Cao J., Xiao L., Xiao J, & Yang G. (2021). Immobilized thrombin on X-ray radiopaque polyvinyl alcohol/chitosan embolic microspheres for precise localization and topical blood coagulation. Bioactive Materials, 6(7), 2105-2119.

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Synthesis of Cellulose Graft Copolymers

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Avicel powder with a degree of polymerization of 200 was provided by Sinopharm Chemical Reagent Co., Ltd, PR China. The monomer ε-caprolactone with a purity of 99.9% used in the preparation of cellulose graft copolymers was obtained from Shandong Xiya Chemical Co., Ltd, PR China, and was used as received without any further purification. The tetrabutylammonium acetate (TBAA) (≥90.0%) and tetrabutylammonium fluoride hydrate (TBAF·3H₂O) (≥98.0%) used as solvent for the cellulose solubilization were purchased from Tokyo Chemical Industry Co., Ltd, Japan. The dimethyl sulfoxide used as assistant solvent for the dissolution of cellulose was of analytical reagent grade and obtained from Beijing Chemical Works, PR China. 4-Dimethylaminopyridine (DMAP) and tin(ii)ethylhexanoate of ≥95% and ≥99% purity, respectively, were supplied by Shanghai Macklin Biochemical Co., Ltd, PR China.

Yu Y., Gao X., Jiang Z., Zhang W., Ma J., Liu X, & Zhang L. (2018). Homogeneous grafting of cellulose with polycaprolactone using quaternary ammonium salt systems and its application for ultraviolet-shielding composite films. RSC Advances, 8(20), 10865-10872.

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Synthesis of Styrene-Itaconic Copolymer

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N-vinyl pyrrolidone (VP,99.5%, Shanghai Yuking Tech, Shanghai, China); styrene (St, 99%, Shanghai Macklin, Shanghai, China) after distillation under reduced pressure and purification; itaconic anhydride (ITA, 99.5%, Shanghai Macklin, Shanghai, China); 2,2′-azobis[2-methyl-n-(2-hydroxyethyl)propionamide) (VA-086), azodiisobutyronitrile (AIBN),β-mercaptoethanol (β-MCE), isopropyl alcohol (IPA), N,N′-Dicyclohexylcarbodiimide (DCC), 4-Dimethylaminopyridine (DMAP), acetone, dioxane, 1-dodecanethiol, carbon disulfide, 2-bromine propionic acid, sodium hydroxide, ethyl alcohol, AlN powder (original particle size: approximate 600 nm) were analytical reagents, from Shanghai Macklin Biochemical Co., Ltd. (Shanghai, China).

Wang Y., Wang S., Zhu G., Xie J., Chen Z, & Shi Y. (2022). Optimizing Hydrolysis Resistance and Dispersion Characteristics via Surface Modification of Aluminum Nitride Powder Coated with PVP-b-P(St-alt-ITA) Copolymer. Molecules, 27(8), 2457.

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Synthesis and Characterization of Boronic Acid-Dextran Conjugate

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4-(Hydroxymethyl) phenylboronic acid pinacol ester (PBAP), dextran with molecular weight (MW) of 5000 g/mol, and ferrous perchlorate were purchased from Shanghai Aladdin Biochemical Technology Co., Ltd (Shanghai, China). 4-Dimethylaminopyridine (DMAP) and NaClO were purchased from Shanghai Macklin Biochemical Co., Ltd (Shanghai, China). N,N’-Carbonyldiimidazole (CDI) was purchased from J&K Chemical Co., Ltd. (Shanghai, China). RAP was purchased from Dalian Meilun Biotechnology Co., Ltd (Dalian, China). Deuterated dimethyl sulfoxide (DMSO-d₆) and deuterated methanol (methanol-d₄) were purchased from Sun Chemical Technology Co., Ltd (Shanghai, China). Hydrogen peroxide (H₂O₂) was purchased from ChengDu Chron Chemicals Co., Ltd (Chengdu, China). DAPI and cell total protein extraction kits were purchased from Beyotime Institute of Biotechnology Co., Ltd (Jiangsu, China). 1,19-Dioctadecyl-3,3,39,39-tetramethylindodicarbocyanine perchlorate (DiD) was purchased from Biotium Inc. (Fremont, USA). The CellTiter 96^TM AQueous One Solution Cell Proliferation Assay (MTS) was purchased from Thermo Fisher Scientific (SanJose, CA, USA).

Tang D., Wang Y., Wijaya A., Liu B., Maruf A., Wang J., Xu J., Liao X., Wu W, & Wang G. (2021). ROS-responsive biomimetic nanoparticles for potential application in targeted anti-atherosclerosis. Regenerative Biomaterials, 8(4), rbab033.

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Synthesis of Amino-Functionalized Silica Nanoparticles

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NH₃·H₂O (AR, 25–28%) was purchased from Xi’an Sanpu Chemical Reagent Co., Ltd. (Xi’an, China). Aminopropyltriethoxysilane (APTES) was purchased from Energy Chemical (Shanghai, China). EGTA, trimethyl [3-(trimethoxysilyl) propyl] ammonium chloride (TMA) (≈50% methanol), TPP, N,N-dicyclohexyl carbonate diimide (DCC), 4-dimethylaminopyridine (DMAP), 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydro (EDC), N-hydroxysuccinimide (NHS), and 1,3,5-trimethylbenzene (TMB) were obtained from Shanghai Macklin Biochemical Co. Tetraethylorthosilicate (TEOS, 99.0%), anhydrous tetrahydrofuran (THF) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Xi’an, China). Hexadecyl trimethyl ammonium bromide (CTAB) and PEG (4000) was purchased from Tianjin Kemiou Chemical Reagent Co., Ltd. (Tianjin, China). Dimethyl sulfoxide (DMSO), toluene, hydrochloric acid, and sodium hydroxide were purchased from Tianjin HengXing Chemical Reagent Co., Ltd. (Tianjin, China). 3-bromopropionic acid was purchased from Shanghai Aladdin Bio-Chem Technology Co., LTD (Shanghai, China).

Lei X., Tan G., Wang Y., Chen L., Cao Y., Si B., Zhen Z., Li B., Jin Y., Wang W, & Jin F. (2023). Mitochondrial Calcium Nanoregulators Reverse the Macrophage Proinflammatory Phenotype Through Restoring Mitochondrial Calcium Homeostasis for the Treatment of Osteoarthritis. International Journal of Nanomedicine, 18, 1469-1489.

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Engineered Multifunctional Theranostic Nanoparticles

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OA was distilled from Camellia seed oil in our lab with its purity of 98% [31 (link),32 (link)]. PEI with molecular weight ≈10,000, mPEG with molecular weight ≈2000, DOX, succinic anhydride, N-hydroxysuccinimide (NHS), 4-Nitrophenyl Chloroformate, o-nitrobenzyl alcohol, 4-dimethylaminopyridine (DMAP), and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC) were purchased from Macklin Reagent Co. (Shanghai, China). Yttrium oxide (Y₂O₃, 99.99%), ytterbium oxide (Yb₂O₃, 99.99%), erbium oxide (Er₂O₃, 99.99%), and sodium fluoride (NaF, 98%) were purchased from Shanghai Chemical Industrial Company (Shanghai, China). Pyrene, 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenytetrazolium bromide (MTT), and 4,6-diamidino-2-phenylindole (DAPI) were purchased from Aladdin Reagent Co. (Shanghai, China). Phosphotungstic acid dye, glutaraldehyde, and copper mesh were purchased from Beijing Zhongjingkeyi Technology Co. (Beijing, China). Human lung adenocarcinoma cells A549 cells and DMEM medium were bought from Shanghai Cell Bank (Shanghai, China). Chloroform, acetonitrile, ethyl ether, and dimethyl sulfoxide (DMSO) were supplied by Sinopharm Chemical Reagent Co. (Shanghai, China). Transparent polystyrene 6-well and 96-well plates were obtained from Corning Co. (Corning, NY, USA).

Zhang J., Tang X., Huang C., Liu Z, & Ye Y. (2020). Oleic Acid Copolymer as A Novel Upconversion Nanomaterial to Make Doxorubicin-Loaded Nanomicelles with Dual Responsiveness to pH and NIR. Pharmaceutics, 12(7), 680.

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