Apact 4004 aggregometer

Manufactured by ELITechGroup

Sourced in France

The APACT-4004 is an aggregometer designed for the measurement of platelet aggregation. It is a laboratory instrument used to analyze the clumping or aggregation of platelets in a blood sample. The device provides quantitative measurement of platelet function and is commonly used in clinical and research settings.

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Lab products found in correlation

Arachidonic acid, by Nu-Chek Prep (2 mentions) Epinephrine, by Merck Group (1 mentions) Ristocetin, by Stago (1 mentions)

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APACT 4004 (9 citations)

5 protocols using apact 4004 aggregometer

Platelet Function Assessment by PRP Analysis

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Platelet rich plasma (PRP) was obtained from blood collected into buffered 3.2% sodium citrate.
Light Transmission Aggregometry (LTA) was evaluated within 4 hours of blood collection using a SD-Soderel aggregometer or APACT-4004 aggregometer (Elitech, France) and different specific agonists that included: 1.25 and 5 μg/ml collagen; 3, 5 and 10 μM ADP; 1 mM arachidonic acid; and 0.75 and 1.25 mg/l ristocetin or Thrombin Receptor Agonist Peptide (TRAP). Percentage maximal amplitude was evaluated using an internal reference interval.
ATP secretion from platelets was tested by a lumiaggregometer assay as recommended by the instrument manufacturer (Chrono-Log Corporation, USA) using PRP, firefly luciferase reagent and 10 μmol/l TRAP, ADP or collagen 14 as agonists.
High-performance liquid chromatography (HPLC) with electrochemical and amperometric detection was used to measure intra-platelet serotonin (5-HT) concentration in PRP samples.

Pennamen P., Le L., Tingaud-Sequeira A., Fiore M., Bauters A., Van Duong Béatrice N., Coste V., Bordet J.C., Plaisant C., Diallo M., Michaud V., Trimouille A., Lacombe D., Lasseaux E., Delevoye C., Picard F.M., Delobel B., Marks M.S, & Arveiler B. (2020). BLOC1S5 pathogenic variants cause a new type of Hermansky-Pudlak Syndrome.. Genetics in medicine : official journal of the American College of Medical Genetics, 22(10), 1613-1622.

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Platelet Aggregation Assay Protocol

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Platelet aggregation was tested in citrated platelet-rich plasma (PRP) using 2.5 μM adenosine diphosphate (ADP; Calbiochem), 1 mM arachidonic acid (Nu Chek Prep), 1 μg/mL Horm equine tendon collagen (Nycomed, Pharma), 10 μM Thrombin Receptor Activating Peptide-14 (TRAP14-mer; Neosystem SA), 4 μM epinephrine (Sigma-Aldrich), and 5 μM ionophore 23187 (Calbiochem) in an APACT-4004 aggregometer (Elitech) according to standard procedures [13 ]. Native PRP concentration was adjusted if the platelet count was higher than 600G/L to reach a platelet count of 500 G/L.

Guy A., Helzy K., Mansier O., Bordet J.C., Rivière E., Fiore M, & James C. (2023). Platelet function studies in myeloproliferative neoplasms patients with Calreticulin or JAK2V617F mutation. Research and Practice in Thrombosis and Haemostasis, 7(2), 100060.

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Platelet Aggregation Assay by LTA

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Platelets aggregation was assessed by light transmittance aggregometry (LTA) on a four-channel APACT 4004 aggregometer (Elitech, France). To obtain homologous Platelet-Poor Plasma (PPP) as negative control, 1.8 mL of PRP was centrifuged at 2500× g during 10 min. Maximal (peak) platelet aggregation (%) induced by ADP 5 µM, ADP 2.5 µM, collagen 3.3 µg/mL, ristocetin 1.25 mg/mL, arachidonic acid 0.5 mg/mL was measured in PRP.

Mayoly A., Iniesta A., Curvale C., Kachouh N., Jaloux C., Eraud J., Vogtensperger M., Veran J., Grimaud F., Jouve E., Casanova D., Sabatier F., Legré R, & Magalon J. (2019). Development of Autologous Platelet-Rich Plasma Mixed-Microfat as an Advanced Therapy Medicinal Product for Intra-Articular Injection of Radio-Carpal Osteoarthritis: From Validation Data to Preliminary Clinical Results. International Journal of Molecular Sciences, 20(5), 1111.

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Platelet Aggregation Testing in Citrated PRP

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Platelet aggregationwas tested in citrated platelet-rich plasma (PRP) using 1.2 mg/mL ristocetin (Stago, Asnières-sur-Seine, France), 5-μM adenosine diphosphate (ADP) (Sigma Aldrich Chimie, Lyon, France), 1-mM arachidonic acid (AA; Nu Chek Prep, Elysian, MN), 25-μM thrombin receptor activating peptide (TRAP; NeosystemSA, Strasbourg, France), 4-μMepinephrine (Helena Laboratories, Beaumont, TX), and 2 μg/μL Horm equine tendon collagen (Nycomed Pharma, Unterschleibheim, Germany) in an APACT 4004 aggregometer (Elitech, Salon de Provence, France) according to standard procedures15 and during 300 seconds of aggregation test time. In the case of normal platelet counts, test results were compared with reference values of 30 healthy controls. Alternatively, when patients had thrombocytopenia, platelet aggregation testswere visually analyzed by hematology experts to confirm the presence of an abnormal profile.

Selle F., James C., Tuffigo M., Pillois X., Viallard J.F., Alessi M.C, & Fiore M. (2017). Clinical and Laboratory Findings in Patients with δ-Storage Pool Disease: A Case Series. Seminars in thrombosis and hemostasis, 43(1).

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Platelet Aggregation Assay Protocol

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Blood cell counts were recorded. Platelet-rich plasma (PRP) and platelet-poor plasma were prepared following the SSC/ISTH recommendations. PRP platelet count lower than 150 × 10 ^9 l -1 and higher than 600 × 10 ^9 l -1 were excluded [10] (link). Platelet aggregation was assessed using an APACT4004 aggregometer (Elitech, Puteaux, France) by light transmission over 10 min after adding adenosine diphosphate (ADP) 10 μM, ristocetin 1.5 mg/ml (Elitech, Puteaux, France), Horm collagen 2 μg/ml (Stago, Taverny, France) or thrombin receptor activating peptide (TRAP-6mer) 10 μM (Bachem, Bubendorf, Switzerland). Percentage of maximal intensity aggregation and maximal disaggregation after addition of agonists were determined from the recordings [9] .

Pourtau L., Sellal J.M., Lacroix R., Poncelet P., Bernus O., Clofent-Sanchez G., Hocini M., Haïssaguerre M., Dignat-George F., Sacher F, & Nurden P. (2017). Platelet function and microparticle levels in atrial fibrillation: Changes during the acute episode. International journal of cardiology, 243.

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