Single bead containing fluorescently tagged red cells was identified using a fluorescent microscope under 10X objective magnification and removed manually with a 20 μl pipette with medium size pipette tips. Selected beads were washed three times with 1.0% SDS and boiled in the same solution for 10 minutes to strip off bound cells and proteins. Finally, the beads were washed three times with water. To cleave the compound from the bead and prepare it for MS/MS sequencing. To prepare the cleaving solution: 30 μl of cyanogen bromide (CNBr) (5.0 M in Acetonitrile (ACN)) was added to 1.0 mL of 0.1 N HCl. 50 μl of cleaving solution was added to the 1.5 mL tube, which contained the single isolated bead. The tube was incubated at 25°C for 4.0 hours. The solution was evaporated using a freeze dryer (SP Scientific, NY, USA), and the cleaved compound was suspended in 20 μL of water. MS/MS sequencing data was obtained using AB Sciex TOF/TOF 5800 machine.
Tof tof 5800 machine
Manufactured by AB Sciex
The TOF/TOF 5800 is a mass spectrometry instrument manufactured by AB Sciex. It is a tandem time-of-flight (TOF/TOF) mass spectrometer designed for the analysis of complex samples. The instrument is capable of performing high-resolution mass analysis and tandem mass spectrometry (MS/MS) experiments.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using tof tof 5800 machine
1
Compound Extraction from Single Bead
2
Single Bead Purification and MS/MS Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
A single bead containing fluorescently tagged red cells was identified using a fluorescent microscope under 10× objective magnification and removed manually with a 20‐μL pipette with medium‐sized pipette tips. Selected beads were washed three times with 1.0% SDS and boiled in the same solution for 45 min to strip off bound cells and proteins. Finally, the beads were washed three times with water. To cleave the compound from the bead and prepare it for MS/MS sequencing, a cleaving solution was prepared, for which 30 μL of CNBr (5.0 m in ACN) was added to 1.0 mL of 0.1 N HCl. 50 μL of the cleaving solution was added to the 1.5‐mL tube containing the single isolated bead. The tube was incubated at 25°C for 4 h. The solution was evaporated using a freeze dryer (SP Scientific, USA), and the cleaved compound was suspended in 20 μL of water. MS/MS sequencing data were obtained using an AB Sciex TOF/TOF 5800 machine.
3
Fluorescent Bead Purification and MS/MS Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Single bead containing fluorescently tagged red cells was identified using a fluorescent microscope under 10× objective magnification and removed manually with a 20 μL pipette with medium size pipette tips. Selected beads were washed three times with 1.0% SDS and boiled in the same solution for 10 min to strip off bound cells and proteins. Finally, the beads were washed three times with water. To cleave the compound from the bead and prepare it for MS/MS sequencing, cleaving solution was prepared; thus, 30 μL of cyanogen bromide (CNBr) (5.0 M in Acetonitrile (ACN)) was added to 1.0 mL of 0.1 N HCl. Cleaving solution (50 μL) was added to the 1.5 mL tube, which contained the single isolated bead. The tube was incubated at 25 °C for 4.0 h. The solution was evaporated using a freeze dryer (SP Scientific, Gardiner, NY, USA), and the cleaved compound was suspended in 20 μL of water. MS/MS sequencing data was obtained using AB Sciex TOF/TOF 5800 machine.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!