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Triglyceride liquid reagents kit

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The Triglyceride Liquid Reagents Kit is a laboratory equipment product designed to measure the concentration of triglycerides in biological samples. It provides the necessary reagents and materials to perform this specific analytical test.

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Lab products found in correlation

Infinity alt kit, by Thermo Fisher Scientific (16 mentions) Ethanol assay kit, by Abcam (15 mentions) Mouse total bile acid kit, by Crystal Chem (4 mentions) Cholesterol liquid reagents kit, by Horiba (3 mentions) Chloramine t, by Merck Group (2 mentions) Versamax microplate reader, by Molecular Devices (2 mentions) Ehrlich s perchloric acid solution, by Thermo Fisher Scientific (2 mentions) Whatman, by Cytiva (2 mentions) Proinflammatory panel 1 mouse kit, by Mesoscale (1 mentions) Ifabp elisa kit, by MyBioSource (1 mentions) Human zonulin elisa kit, by MyBioSource (1 mentions) Oil red o staining, by Merck Group (1 mentions) Quantichrom alkaline phosphatase assay kit, by BioAssay Systems (1 mentions) Hydroxyproline colorimetric assay kit, by Abcam (1 mentions) Mouse il 22 duoset elisa kit, by R&D Systems (1 mentions) Mouse lipopolysaccharides elisa kit, by Cusabio (1 mentions)

Spelling variants of this product

Triglyceride Reagent (12 citations) Triglycerides Reagent (4 citations) Triglycerides Liquid Reagents (3 citations) Triglyceride Reagent Kit (3 citations)

21 protocols using triglyceride liquid reagents kit

1

Measuring Hepatic and Fecal Lipids in Mice

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Hepatic triglyceride levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific). For fecal triglyceride quantitation, fecal samples were mixed with phosphate-buffered saline (PBS) in a 1:40 dilution and centrifuged at 12,000 g for 5 min to separate solid particles. Triglyceride concentrations in supernatants were determined as previously described employing the Triglyceride Liquid Reagents Kit (Pointe Scientific) [13 (link)]. Hepatic cholesterol levels were measured using the Cholesterol Liquid Reagents Kit (Pointe Scientific). Total serum bile acids were measured using a Mouse Total Bile Acid kit (Crystal Chem). Plasma lipopolysaccharide (LPS) was quantitated using an ELISA Kit for Lipopolysaccharides (LPS) (Lifeome Biolabs). For determination of tissue hydroxyproline content, liver specimens (100 mg) were homogenized in 6 N HCl (3750–32; USABlueBook), using lysing matrix C tubes with the Mini-BeadBeater-96, and subsequently incubated at 110°C for 24 hours. The lysate was filtered using Whatman filter paper, grade 595 1/2 (WHA10311644; Sigma Aldrich). After incubation with chloramine T– (C9887; Sigma Aldrich) and Ehrlich’s perchloric acid solution (AC168760250; Thermo Fisher Scientific), samples were measured at 558 nm (VersaMax Microplate Reader; Molecular Devices LLC, Sunnyvale, CA).
Hartmann P., Duan Y., Miyamoto Y., Demir M., Lang S., Hasa E., Stern P., Yamashita D., Conrad M., Eckmann L, & Schnabl B. (2022). Colesevelam ameliorates non-alcoholic steatohepatitis and obesity in mice. Hepatology International, 16(2), 359-370.
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2

Quantitative Analysis of Hepatic and Fecal Lipid Profiles

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Hepatic triglyceride levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific). For fecal triglyceride quantitation, fecal samples were mixed with phosphate-buffered saline (PBS) in a 1:40 dilution and centrifuged at 12,000 g for 5 min to separate solid particles. Triglyceride concentrations in supernatants were determined as previously described employing the Triglyceride Liquid Reagents Kit (Pointe Scientific) [13 (link)]. Hepatic cholesterol levels were measured using the Cholesterol Liquid Reagents Kit (Pointe Scientific). Total serum bile acids were measured using a Mouse Total Bile Acid kit (Crystal Chem). Plasma lipopolysaccharide (LPS) was quantitated using an ELISA Kit for Lipopolysaccharides (LPS) (Lifeome Biolabs). For determination of tissue hydroxyproline content, liver specimens (100 mg) were homogenized in 6 N HCl (3750‐32; USABlueBook), using lysing matrix C tubes with the Mini‐BeadBeater‐96, and subsequently incubated at 110 °C for 24 h. The lysate was filtered using Whatman filter paper, grade 595 1/2 (WHA10311644; Sigma-Aldrich). After incubation with chloramine T– (C9887; Sigma-Aldrich) and Ehrlich's perchloric acid solution (AC168760250; Thermo Fisher Scientific), samples were measured at 558 nm (VersaMax Microplate Reader; Molecular Devices LLC, Sunnyvale, CA).
Hartmann P., Duan Y., Miyamoto Y., Demir M., Lang S., Hasa E., Stern P., Yamashita D., Conrad M., Eckmann L, & Schnabl B. (2022). Colesevelam ameliorates non-alcoholic steatohepatitis and obesity in mice. Hepatology International, 16(2), 359-370.
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3

Biochemical Profiling of Liver Function

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Serum levels of ALT were determined using Infinity ALT kit (Thermo Scientific). Hepatic triglyceride levels were measured using Triglyceride Liquid Reagents kit (Pointe Scientific). Levels of serum LPS and fecal albumin were determined by ELISA kits (Lifeome Biolabs and Bethyl Labs, respectively). Serum levels of ethanol were measured using Ethanol Assay kit (BioVision).
Duan Y., Llorente C., Lang S., Brandl K., Chu H., Jiang L., White R.C., Clarke T.H., Nguyen K., Torralba M., Shao Y., Liu J., Hernandez-Morales A., Lessor L., Rahman I.R., Miyamoto Y., Ly M., Gao B., Sun W., Kiesel R., Hutmacher F., Lee S., Ventura-Cots M., Bosques-Padilla F., Verna E.C., Abraldes J.G., Brown RS J.r., Vargas V., Altamirano J., Caballería J., Shawcross D.L., Ho S.B., Louvet A., Lucey M.R., Mathurin P., Garcia-Tsao G., Bataller R., Tu X.M., Eckmann L., van der Donk W.A., Young R., Lawley T.D., Stärkel P., Pride D., Fouts D.E, & Schnabl B. (2019). Bacteriophage targeting of gut bacterium attenuates alcoholic liver disease. Nature, 575(7783), 505-511.
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4

Biochemical Profiling of Liver Function

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Serum levels of ALT were determined using Infinity ALT kit (Thermo Scientific). Hepatic triglyceride levels were measured using Triglyceride Liquid Reagents kit (Pointe Scientific). Levels of serum LPS and fecal albumin were determined by ELISA kits (Lifeome Biolabs and Bethyl Labs, respectively). Serum levels of ethanol were measured using Ethanol Assay kit (BioVision).
Duan Y., Llorente C., Lang S., Brandl K., Chu H., Jiang L., White R.C., Clarke T.H., Nguyen K., Torralba M., Shao Y., Liu J., Hernandez-Morales A., Lessor L., Rahman I.R., Miyamoto Y., Ly M., Gao B., Sun W., Kiesel R., Hutmacher F., Lee S., Ventura-Cots M., Bosques-Padilla F., Verna E.C., Abraldes J.G., Brown RS J.r., Vargas V., Altamirano J., Caballería J., Shawcross D.L., Ho S.B., Louvet A., Lucey M.R., Mathurin P., Garcia-Tsao G., Bataller R., Tu X.M., Eckmann L., van der Donk W.A., Young R., Lawley T.D., Stärkel P., Pride D., Fouts D.E, & Schnabl B. (2019). Bacteriophage targeting of gut bacterium attenuates alcoholic liver disease. Nature, 575(7783), 505-511.
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5

Biochemical Markers of Hepatic Injury

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Serum levels of ALT were determined using Infinity ALT kit (Thermo Scientific). Hepatic triglyceride levels were measured using Triglyceride Liquid Reagents kit (Pointe Scientific). Serum levels of ethanol were measured using Ethanol Assay kit (BioVision). Serum levels of cytokines were measured using Proinflammatory Panel 1 (mouse) kit (Meso Scale Diagnostics). All results were measured using the software SoftMax Pro 7.0.3.
Duan Y., Chu H., Brandl K., Jiang L., Zeng S., Meshgin N., Papachristoforou E., Argemi J., Mendes B.G., Wang Y., Su H., Sun W., Llorente C., Hendrikx T., Liu X., Hosseini M., Kisseleva T., Brenner D.A., Bataller R., Ramachandran P., Karin M., Fu W, & Schnabl B. (2021). CRIg on liver macrophages clears pathobionts and protects against alcoholic liver disease. Nature Communications, 12, 7172.
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6

Evaluating Liver and Intestinal Markers

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Serum levels of ALT were measured using the Infinity ALT kit (Thermo Scientific). Hepatic triglyceride levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific). Serum levels of ethanol were measured using the Ethanol Assay Kit (BioVision). Serum levels of intestinal fatty-acid binding protein (IFABP) were measured using the iFABP ELISA kit (MyBioSource). Serum levels of zonulin were measured using the Human Zonulin ELISA Kit (MyBioSource)17 .
Chu H., Duan Y., Lang S., Jiang L., Wang Y., Llorente C., Liu J., Mogavero S., Bosques-Padilla F., Abraldes J.G., Vargas V., Tu X.M., Yang L., Hou X., Hube B., Stärkel P, & Schnabl B. (2019). The Candida albicans exotoxin Candidalysin promotes alcohol-associated liver disease. Journal of hepatology, 72(3), 391-400.
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7

Quantifying Liver and Bile Metabolites

Cited 1 time
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Levels of plasma ALT were measured using the Infinity ALT kit (Thermo Scientific, Waltham, MA). Plasma and hepatic triglyceride and cholesterol levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific, Canton, MI) and Cholesterol Liquid Reagents kit (Pointe Scientific), respectively. Plasma levels of leptin were measured using the mouse leptin enzyme-linked immunosorbent assay kit according to the manufacturer’s protocol (Crystal Chem, Elk Grove Village, IL). Total bile acids and the bile acid pool were quantified using a Mouse Total Bile Acid Kit (Crystal Chem) as described.59 (link) For the total bile acid pool, total liver bile acids, total gallbladder bile acids, and total bile acids from the small intestine and cecum, contents were measured and calculated per gram of body weight.
Zhou R., Llorente C., Cao J., Zaramela L.S., Zeng S., Gao B., Li S.Z., Welch R.D., Huang F.Q., Qi L.W., Pan C., Huang Y., Zhou P., Beussen I., Zhang Y., Bryam G., Fiehn O., Wang L., Liu E.H., Yu R.T., Downes M., Evans R.M., Goglin K., Fouts D.E., Brenner D.A., Bode L., Fan X., Zengler K, & Schnabl B. (2021). Intestinal α1-2-Fucosylation Contributes to Obesity and Steatohepatitis in Mice. Cellular and Molecular Gastroenterology and Hepatology, 12(1), 293-320.
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8

Comprehensive Metabolic Assessment in Mouse Models

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Plasma levels of ethanol were measured using the Ethanol Assay Kit (BioVision). Levels of ALT were measured using Infinity ALT kit (Thermo Scientific). Hepatic triglyceride levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific). Hepatic alcohol dehydrogenase (ADH) activity was measured using the ADH Assay Kit (BioVision). Plasma LPS (Cloud-Clone Corp), and fecal albumin levels (Bethyl lab) were measured by ELISA. Whole blood was collected into potassium EDTA–containing Microvette 100 tubes (Sarstedt), and automated complete blood counts (CBCs) were obtained using a Scil Vet abc automatic hematology analyzer (Scil Animal Care). For insulin tolerance test (ITT), following a 6-hour fasting period, an intraperitoneal dose of 0.35 or 1 U kg−1 insulin (novolin, Novo Nordisk Inc.) was administered to mice on RC diet or HFD, respectively, and blood glucose was measured at indicated time points. Plasma and liver levels of iron were measured using a kit from Thermo Scientific.
Llorente C., Jepsen P., Inamine T., Wang L., Bluemel S., Wang H.J., Loomba R., Bajaj J.S., Schubert M.L., Sikaroodi M., Gillevet P.M., Xu J., Kisseleva T., Ho S.B., DePew J., Du X., Sørensen H.T., Vilstrup H., Nelson K.E., Brenner D.A., Fouts D.E, & Schnabl B. (2017). Gastric acid suppression promotes alcoholic liver disease by inducing overgrowth of intestinal Enterococcus. Nature Communications, 8, 837.
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9

Plasma Biomarker Quantification

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Levels of plasma alanine amino-transferase (ALT) were measured using infinity ALT kit (Thermo Fisher Scientific, Waltham, MA). Triglyceride levels were measured using the Triglyceride Liquid Reagents Kit (Pointe Scientific, Canton, MI). Levels of plasma ethanol were measured using the Ethanol Assay Kit (Bio Vision, Milpitas, CA).
Zhou R., Llorente C., Cao J., Gao B., Duan Y., Jiang L., Wang Y., Kumar V., Stärkel P., Bode L., Fan X, & Schnabl B. (2020). Deficiency of Intestinal α1-2-fucosylation Exacerbates Ethanol-Induced Liver Disease in Mice. Alcoholism, clinical and experimental research, 44(9), 1842-1851.
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10

Hepatic Biomarkers in Mice

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Mouse serum ALT levels were measured by ALT (SGPT) Kinetic (Teco Diagnostics). Mouse hepatic triglyceride levels were determined using Triglyceride Liquid Reagents kit (Pointe Scientific). Mouse serum levels of ethanol were measured using an Ethanol Assay kit (Abcam).
Zeng S., Hartmann P., Park M., Duan Y., Lang S., Llorente C., Wang Y., Cabré N., Fouts D.E., Bacher P., Jung W.H., Stärkel P, & Schnabl B. (2023). Malassezia restricta promotes alcohol-induced liver injury. Hepatology Communications, 7(2), e0029.
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