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4 protocols using β pinene

1

Essential Oil Compounds Procurement

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Thymol methyl ether, Carvacrol methyl ether, (+)-2-carene, 1-octen-3-ol, myrcene, trans-anethole, α-terpineol were purchased from Sigma-Aldrich (St. Louis, MO, USA). Carvacrol, γ-terpinene, 3-carene, p-cymene, camphene, α-terpinene, terpinolene, (+/−)-α-pinene, (−/−)-β-pinene, R-(+)-limonene, S-(−)-limonene, were purchased from Extrasynthese (Genay, France). Thymol, linalool, α-phellandrene were purchased from Merck Millipore (Boston, MA, USA). All reagents and solvents have European Pharmacopoeia quality.
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2

Cholinesterase Inhibition by Essential Oils

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C7-C40 saturated alkane standard, acetylthiocholine iodide (ATCI), 5,5′-dithiobis (2-nitrobenzoic acid) (DTNB), AChE, galanthamine, sodium phosphate dibasic (Na2HPO4), potassium phosphate monobasic (KH2PO4), sodium chloride (NaCl) and potassium chloride (KCl) were purchased from Sigma-Aldrich (Milan, Italy). α-Pinene, camphene, β-pinene, limonene, 1-8-cineole, linalool, borneol, terpinen-4-ol, citronellol, bornyl acetate, β-caryophyllene, aromadendrene, α-humulene and guaiol were purchased from Extrasynthese (Genay, France). Dichloromethane was GC-grade and was purchased from Merck (Darmstadt, Germany). All other chemicals and solvents were of analytical grade.
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3

Cannabinoid and Terpene Standard Analysis

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CBD standard solution (1 mg/mL in methanol) was purchased from Cerilliant (Round Rock, TX, USA). α-Pinene, β-pinene, β-myrcene, α-terpinolene and β-caryophyllene were purchased from Extrasynthese (Genay, France). n-Hexane GC-grade was purchased from VWR (Milan, Italy).
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4

Antioxidant and Enzymatic Assays

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Folin-Ciocalteu phenol reagent, 2,2-diphenyl-1-picrylhydrazyl (DPPH), potassium peroxydisulfate, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS •+ ), 2-4-6-tris(2piridil)-s-triazina (TPTZ), ethylendiaminetetracetic acid (EDTA), iron sulphate heptahydrate, ferrozine, sodium phosphate dibasic, potassium phosphate monobasic, sodium acetate, iron(III) chloride hexahydrate, iron(II) chloride tetrahydrate, 2,2'-Azobis(2-amidinopropane) dihydrochloride (AAPH), fluorescein disodium salt, C 7 -C 40 saturated alkane standard, linalyl anthranilate, acetylthiocholine iodide (ATCI), and 5,5'-dithiobis(2-nitrobenzoic acid) (DTNB) were purchased from Sigma-Aldrich (Milan, Italy). D-Limonene, Linalool, β-Pinene and α-Terpineol were purchased from Extrasynthese (Genay, France). Dichloromethane was GC-grade and was purchased from Merck (Darmstadt, Germany). All Other chemicals and solvents were of analytical grade.
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