Biotinylated aleuria aurantia lectin

Manufactured by Vector Laboratories

Biotinylated Aleuria Aurantia Lectin is a glycoprotein isolated from the fungus Aleuria aurantia. It has a high affinity for specific carbohydrate structures and is commonly used as a reagent for the detection and labeling of those structures in biological samples.

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Lab products found in correlation

Avidin and biotin, by Vector Laboratories (1 mentions) Picrosirius red, by Merck Group (1 mentions) 3 3 diaminobenzidine solution, by Vector Laboratories (1 mentions) Ulex europaeus agglutinin 1, by Vector Laboratories (1 mentions) Ab6556, by Abcam (1 mentions)

Spelling variants of this product

Biotinylated lectins (43 citations) Aleuria aurantia lectin (AAL) (22 citations) Lectins (7 citations)

2 protocols using biotinylated aleuria aurantia lectin

Histological Evaluation of Liver and Colon Tissues

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Formalin-fixed and paraffin-embedded livers were stained with H&E (Leica Biosystems, Inc, Buffalo Grove, IL) or 0.1% picrosirius red (Sigma-Aldrich) using standard staining protocols. Sirius red–positive area was quantitated by image analysis software ImageJ (National Institutes of Health, Bethesda, MD). The colon sections were treated with 0.1% H₂O₂ (Sigma-Aldrich) for 30 minutes and blocked with avidin and biotin (Vector, Torrance, CA) for 15 minutes each. After blocking with 1% bovine serum albumin for 5 minutes, colon sections were incubated with biotinylated Ulex Europaeus Agglutinin I (Vector) or biotinylated Aleuria Aurantia Lectin (Vector) overnight at 4°C. Sections then were incubated with streptavidin, horseradish peroxidase for 30 minutes, and followed by 3, 3’-diaminobenzidine solution (Vector) for 2 minutes and hematoxylin for 1 minute.

Zhou R., Llorente C., Cao J., Zaramela L.S., Zeng S., Gao B., Li S.Z., Welch R.D., Huang F.Q., Qi L.W., Pan C., Huang Y., Zhou P., Beussen I., Zhang Y., Bryam G., Fiehn O., Wang L., Liu E.H., Yu R.T., Downes M., Evans R.M., Goglin K., Fouts D.E., Brenner D.A., Bode L., Fan X., Zengler K, & Schnabl B. (2021). Intestinal α1-2-Fucosylation Contributes to Obesity and Steatohepatitis in Mice. Cellular and Molecular Gastroenterology and Hepatology, 12(1), 293-320.

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Immunoprecipitation of GFP-tagged PRRs

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Agrobacteria containing 35S:GFP-PRRs and 35S:SPY-FLAG were coinfiltrated into 5 week-old N. benthamiana leaves. After 3 days, the infiltrated leaves were collected and ground in liquid nitrogen to fine powder. Protein extraction and immunoprecipitation were conducted as former description with GFP antibody (ab11120, Invitrogen) (Wang et al., 2013 (link)). GFP-tagged PRRs and FLAG tagged SPY and its deletions were detected by Western blotting using GFP antibody (ab6556, Abcam) FLAG antibody (M20008, Abmart), and Biotinylated-Aleuria aurantia Lectin (Vector labs, B-1395).

Wang Y., He Y., Su C., Zentella R., Sun T.P, & Wang L. (2019). Nuclear Localized O-Fucosyltransferase SPY Facilitates PRR5 Proteolysis to Fine-Tune the Pace of Arabidopsis Circadian Clock. Molecular plant, 13(3), 446-458.

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