Rabbit anti myc a 14

HeLa cells stably transfected with the indicated plasmids were lysed using RIPA lysis buffer (50 mM Tris–HCl [pH 7.5], 150 mM NaCl, 1 mM EDTA, 1% Triton X-100 supplemented with 1 × cOmplete protease inhibitor cocktail (Sigma-Aldrich). The cell lysates were electrophoresed via SDS–PAGE, transferred onto PVDF membranes (Amersham), blocked with 5% non-fat dried milk (BD Biosciences), and incubated with appropriate primary and HRP-conjugated secondary antibodies (Bio-Rad Laboratories). Immunoblots were visualized using an ECL detection system (Santa Cruz Biotechnology). The Western blots were imaged using ChemiDoc MP (Bio-Rad). The following primary antibodies were used: rabbit anti-Myc (A14; Santa Cruz, 1:1,000), rabbit anti-E-cadherin (24E10; Cell Signaling Technology, 1:1,000), anti-vimentin (D21H3; Cell Signaling, 1:1,000), anti-N-cadherin (#32; BD Biosciences, 1:1,000), anti-β-catenin (6B3; Cell Signaling, 1:1,000), anti-β-tubulin (9F3; Cell Signaling, 1:1,000), and anti-DSG1 (H-290; Santa Cruz, 1:1,000). The full-length blots are shown in Supplementary data, Fig. S13.