Western blot analysis was carried out as described before (Hausrat et al., 2022 (link)). Briefly, all primary antibodies were incubated in TRIS-buffered saline (TBS) supplemented with Tween-20 (TBS-T) containing 5% (w/v) dry milk (Roth, Karlsruhe, Germany) for 1 h at room temperature or, respectively, overnight at 4°C. All secondary antibodies were incubated in TBS-T containing 5% (w/v) dry milk (Roth, Karlsruhe, Germany) for 1 h at room temperature. For detection of relative immunoblot signal intensities, images were acquired using a Chemo-Cam Imager ECL HR 16-3200 (Intas) or an Odyssey CLx (LI-COR) imaging system. Signal intensities were analyzed using Fiji (ImageJ, version 2.0, NIH, United States).
Hausrat T.J., Vogl C., Neef J., Schweizer M., Yee B.K., Strenzke N, & Kneussel M. (2022). Monoallelic loss of the F-actin-binding protein radixin facilitates startle reactivity and pre-pulse inhibition in mice. Frontiers in Cell and Developmental Biology, 10, 987691.
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