Cells were fixed in 4% paraformaldehyde for 10–20 min, washed with PBS three times (5 min each), permeabilized with 0.1% triton X-100 for 15 min, incubated in blocking solution (2% BSA) for 1 hour at RT and then in primary antibodies (goat anti-Nanog, Abcam ab77095, 1:500; rabbit anti-Lin28, Abcam ab46020, 1:500; rabbit anti-Oct4, Abcam ab19857, 1:500; mouse anti-SSEA4, Abcam ab16287, 1:200; mouse anti-Nestin, Abcam ab22035, 1:200; rabbit anti-Musashi1, Abcam ab52865, 1:250; rat anti-CTIP2, Abcam ab18465, 1:250; rabbit anti-SATB2, Abcam ab34735, 1:200; chicken anti-MAP2, Abcam ab5392, 1:1000; rabbit anti-FZD9, Origene TA314730, 1:150; chicken anti-EGFP, Abcam ab13970, 1:1000; rabbit anti-Synapsin1, EMD-Millipore AB1543P, 1:500; mouse anti-Vglut1, Synaptic Systems 135311, 1:500; rabbit anti-Homer1, Synaptic Systems 160003, 1:500) overnight at 4°C. The next day, cells were washed with PBS three times (5 min each), incubated with secondary antibodies (Alexa Fluor 488, 555 and 647, Life Technologies, 1:1000) for 1 hour at RT, and washed with PBS three times (5 min each). Nuclei were stained using DAPI (1:10,000). Slides or coverslips were mounted using ProLong Gold antifade mountant (Life Technologies).
Ta314730
Manufactured by Abcam
TA314730 is a reagent for use in laboratory research applications. It is a purified antibody that can be used to detect and quantify the target protein in samples. The core function of this product is to provide a specific and sensitive tool for researchers to investigate the presence and levels of the target protein in their experiments.
Automatically generated - may contain errors
Lab products found in correlation
Ab13970, by Abcam (2 mentions)
Prolong gold antifade mountant, by Thermo Fisher Scientific (2 mentions)
Ab19857, by Abcam (2 mentions)
Ab52865, by Abcam (2 mentions)
Ab22035, by Abcam (2 mentions)
Ab46020, by Abcam (2 mentions)
Ab77095, by Abcam (2 mentions)
Ab5392, by OriGene (2 mentions)
Ab16287, by Abcam (2 mentions)
Ab34735, by Abcam (2 mentions)
Ab18465, by Abcam (2 mentions)
Ab1543p, by Synaptic Systems (2 mentions)
2 protocols using ta314730
1
Immunostaining of Pluripotent and Neural Markers
2
Immunostaining of Pluripotent and Neural Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were fixed in 4% paraformaldehyde for 10–20 min, washed with PBS three times (5 min each), permeabilized with 0.1% triton X-100 for 15 min, incubated in blocking solution (2% BSA) for 1 hour at RT and then in primary antibodies (goat anti-Nanog, Abcam ab77095, 1:500; rabbit anti-Lin28, Abcam ab46020, 1:500; rabbit anti-Oct4, Abcam ab19857, 1:500; mouse anti-SSEA4, Abcam ab16287, 1:200; mouse anti-Nestin, Abcam ab22035, 1:200; rabbit anti-Musashi1, Abcam ab52865, 1:250; rat anti-CTIP2, Abcam ab18465, 1:250; rabbit anti-SATB2, Abcam ab34735, 1:200; chicken anti-MAP2, Abcam ab5392, 1:1000; rabbit anti-FZD9, Origene TA314730, 1:150; chicken anti-EGFP, Abcam ab13970, 1:1000; rabbit anti-Synapsin1, EMD-Millipore AB1543P, 1:500; mouse anti-Vglut1, Synaptic Systems 135311, 1:500; rabbit anti-Homer1, Synaptic Systems 160003, 1:500) overnight at 4°C. The next day, cells were washed with PBS three times (5 min each), incubated with secondary antibodies (Alexa Fluor 488, 555 and 647, Life Technologies, 1:1000) for 1 hour at RT, and washed with PBS three times (5 min each). Nuclei were stained using DAPI (1:10,000). Slides or coverslips were mounted using ProLong Gold antifade mountant (Life Technologies).
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