Complete freund s adjuvant cfa

Ovalbumin (OVA) was purchased from Sigma. Monosodium urate and basic calcium phosphate crystals, both used at 250 ug/ml for antigen presentation assays, were made from materials from Sigma as described previously [33 (link), 37 (link)]. For MSU, the crude crystal preparation was tumbled with 5 mm stainless steel beads in a 50 ml conical tube for one week to produce finer crystals about 1um in length. Latex beads (3 um) were purchased from Polyscience Inc. Complete Freund’s adjuvant (CFA) and incomplete Freund’s adjuvant (IFA) were from Rockland. All IL-2 ELISA Ready-Set-Go kits were from eBioscience. Alexa-488 OVA, Lysotracker (deep red and green) and Alexa-594 Transferrin receptor were bought from Invitrogen. Anti mouse CD4 was from BD. Anti mouse antibodies for CD8, CD40, CD45.1, CD69, CD80, CD86, CD90.1, MHC I (H2Db), MHC II (Ia/Ie), IL-4 and IFN-γ were from eBioscience Inc. Secondary antibodies and Prolong Gold Antifade were bought from Molecular Probes (Invitrogen).