GC cells were seeded on confocal dishes (Solarbio, Beijing, China) and incubated in proper media for one night. Then the cells were fixed with formaldehyde (Biosharp, Hefei, Anhui, China) and permeabilized with 0.2% TritonX‐100 (Beyotime, Shanghai, China), which followed by blocking with 1% bovine serum albumin (BSA; Beyotime, Shanghai, China) for 30 min. Next, cells were incubated with the primary antibody overnight at 4°C and secondary antibody for 2 h on the next day. Finally, cells were detected with a confocal microscope (Leica, Weszler, Hesse, Germany). The reagents required above were obtained from Beyotime. The antibodies used in this study are listed in Supplementary Table S1 .
Confocal dishes
Manufactured by Solarbio
Sourced in China
Confocal dishes are specialized laboratory containers designed for cell culture and microscopy applications. They feature a thin, optically clear bottom that enables high-resolution imaging using confocal microscopy techniques.
Automatically generated - may contain errors
2 protocols using confocal dishes
1
Immunofluorescence Staining of GC Cells
2
Immunofluorescence Imaging of GC Cells
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3 × 104 GC cells were seeded in the confocal dishes (Solarbio, Beijing, China) on the first day. The next day, the cells were fixed and permeabilized followed by blocking for 2 h. Then the cells were sequentially incubated with primary antibodies overnight and secondary antibodies for 1 h. Finally, the nuclei of cells were stained with DAPI, and LSM 710 confocal microscope (Zeiss, Germany) was used for Image acquisition. Information about the primary and secondary antibodies used in IF are listed in Supplementary Table S6 .
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