Fast 96 well block module

Manufactured by Thermo Fisher Scientific

The Fast 96-Well Block Module is a laboratory equipment designed to provide efficient thermal control for 96-well sample plates. It is compatible with various thermal cyclers and can be used to perform a range of temperature-dependent applications, such as PCR (Polymerase Chain Reaction) and other molecular biology techniques.

Automatically generated - may contain errors

Lab products found in correlation

7900ht fast real time pcr system, by Thermo Fisher Scientific (2 mentions) Rt2 sybr green rox fast mastermix, by Qiagen (1 mentions) Sds plate utility v2, by Thermo Fisher Scientific (1 mentions) Superscript 3 reverse transcriptase, by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

96-well block module (7 citations) 7900HT Fast Real-Time PCR System with Fast 96-Well Block Module (5 citations) 96-block module (4 citations)

2 protocols using fast 96 well block module

Quantitative RT-PCR of Inflammation Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pre-designed TaqMan^® primer assays were purchased from Applied Biosystems (Mrp8, cat. no. Mm00496696_g1; Mrp14, cat. no. Mm00656925_m1; Cd68, cat. no. Mm03047343_m1; Itgb2, cat. no. Mm00434513_m1; Lyz1, cat. no. Mm00657323_m1). RT-PCR reactions were performed using the 7900HT Fast Real-time PCR system with Fast 96-Well Block Module (Applied Biosystems, cat. no. 4351405). Cycle threshold values of target genes from each sample were normalized using 18S ribosomal RNA (Rn18s) as an endogenous control gene (Applied Biosystems, cat. no. Mm04277571_s1). Differential expression analysis and estimation of FC between treatment groups was then performed using the 2^−ΔΔCt method [74 (link)]. RT-PCR analysis with respect to liver samples yielded fold-change estimates consistent with those generated by microarray analysis (Mrp8, Mrp14, Cd68, Itgb2 and Lyz1).

Swindell W.R., Xing X., Fritz Y., Diaconu D., Simon D.I., Ward N.L, & Gudjonsson J.E. (2016). Deficiency of myeloid-related proteins 8 and 14 (Mrp8/Mrp14) does not block inflammaging but prevents steatosis. Oncotarget, 7(24), 35535-35551.

+ Open protocol

+ Expand

RT-qPCR Transcripts Evaluation

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was reverse transcribed using SuperScript III Reverse Transcriptase (Invitrogen, Waltham, MA). Transcripts were evaluated with customized Qiagen RT2 Primers Assays (Valencia, CA), details can be found in Supplementary Methods. PCRs were performed using RT² SYBR Green ROX FAST Mastermix (Qiagen), in a 7900HT Fast Real-Time PCR System with Fast 96-Well Block Module (Applied Biosystems, Foster City, CA) with a SDS Plate utility v2.2 software (Applied Biosystems). The results were normalized to the geometric mean of GAPDH and 18S housekeeping genes.

Kesby J.P., Chang A., Najera J.A., Marcondes M.C, & Semenova S. (2019). Brain Reward Function after Chronic and Binge Methamphetamine Regimens in Mice Expressing the HIV-1 TAT Protein. Current HIV research, 17(2), 126-133.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!