Prontosan wound irrigation solution

The tested antiseptic solutions were:(a)

Prontosan Wound Irrigation Solution (B. Braun Medical AG), later referred to as “P”, which contained 0.1% undecylenamidopropyl betaine, 0.1% polyhexamethylene biguanide (polihexanide), and purified water.

(b)

Octenilin Wound Irrigation Solution, (Schülke Mayr GmbH, Vienna, Austria), later referred to as “O”, which contained Aqua valde purificata, Glycerol, Ethylhexylglycerin and Octenidine HCl.

(c)

Microdacyn 60 Wound Care Solution (Sonoma Pharmaceuticals, Inc, Petaluma, CA, USA), later referred to as “M” which contained super-oxidized water, sodium chloride (0.022%), hypochlorous acid (0.004%), sodium hypochlorite (0.004%).

All antiseptics were provided by B. Braun Medical AG in non-transparent, unsigned bottles containing 250 mL of colorless solutions referred to as “WIS1, WIS2, WIS3”, where “WIS” stood for “wound irrigation solution”. The identity of WIS’s was decrypted after the end of research and introduced to the manuscript of this work.

Prontosan wound irrigation solution (B. Braun; Melsungen, Germany), poviargolum powder (FSUE “SCTB ‘Technolog’”; Saint Petersburg, Russia), Xydifon (commercial name of etidronic acid 20% aqueous solution; OJSC “MosChemPharm” named after N.A. Semashko; Moscow, Russia), and Dioxydin 1% aqueous solution (OJSC “MosChemPharm” named after N.A. Semashko; Moscow, Russia) were purchased from the market. CP grade powders of sodium lauroyl sarcosinate (Sigma-Aldrich, St. Louis, MO, USA) and cocamidopropyl betaine (Evonik Industries, Essen, Germany) were helpfully provided by our colleagues from the Institute of Macromolecular Compounds of RAS and CP grade sodium hypochlorite 10% aqueous solution by our colleagues from the Faculty of Dentistry and Medical Technology of the Saint Petersburg State University.