Following phenotypic AMR characterization, 24 E. coli isolates classified as either ESBL or AmpC phenotypes, resistant to NAL or reduced susceptibility to CIP were selected for WGS. The DNA was extracted using a commercial DNA extraction kit (Qiagen, Venlo, Netherland), libraries were prepared using the Nextera XT DBA library preparation kit (Illumina, California, United States) and the sequencing was performed using an Illumina Miseq (Illumina, California, United States). Generated raw reads (fastq files) were assembled using SPAdes 3.9 on the Center for Genomic Epidemiology platform.
Nextera xt dba library preparation kit
Manufactured by Illumina
Sourced in United States
The Nextera XT DNA Library Preparation Kit is a lab equipment product developed by Illumina. It is designed to facilitate the creation of DNA libraries for next-generation sequencing applications. The kit includes the necessary reagents and protocols to prepare DNA samples for sequencing, enabling users to generate libraries efficiently and consistently.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using nextera xt dba library preparation kit
1
Whole-Genome Sequencing of ESBL and AmpC Resistant E. coli
2
Whole-Genome Sequencing of ESBL/AmpC E. coli
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Following phenotypic AMR characterization, 24 E. coli isolates classified as either ESBL or AmpC phenotypes, resistant to NAL or reduced susceptibility to CIP were selected for WGS. The DNA was extracted using a commercial DNA extraction kit (Qiagen,Venlo, Netherland), libraries were prepared using the Nextera XT DBA library preparation kit (Illumina, California, United States) and the sequencing was performed using an Illumina Miseq (Illumina, California, United States). Generated raw reads (fastq files) were assembled using SPAdes 3.9 on the Center for Genomic Epidemiology platform.
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