The Bombyx mori silkworm fibers were obtained from China (Jiuyuan mulberry silk, Hangzhou, China). For silk degumming, Na2CO3 (Sigma-Aldrich, St. Louis, MO, USA) was used. LiBr (Sigma-Aldrich, St. Louis, MO, USA) was used to dissolve the degummed silk fibers, and cellulose membranes (3500 MWCO, Thermo Scientific, Rockford, IL, USA) were used to dialyze the solution. Gelatin (Sinopharm, Peking, China) solution was used to mix with CSF and form hydrogel, chondrocytes (YaJi Biological, Shanghai, China) were cultured with the mixture of DMEM-F12 (Meilunbio, Dalian, China), fetal bovine serum (Sangon Biotech, Shanghai, China), and antibiotic/antimycotic (Sangon Biotech, Shanghai, China). The hydrogels with encapsulated cells were stained with a live/dead Viability Kit (Life Technologies, Grand Island, NY, USA).
Antibiotic antimycotic
Manufactured by Sangon
Sourced in China
Antibiotic/antimycotic is a sterile solution containing a combination of antibiotics and antifungal agents. It is commonly used in cell culture applications to prevent bacterial and fungal contamination.
Automatically generated - may contain errors
Lab products found in correlation
Fetal bovine serum (fbs), by Sangon (2 mentions)
3500 mwco, by Thermo Fisher Scientific (1 mentions)
Live dead viability kit, by Thermo Fisher Scientific (1 mentions)
Na2co3, by Merck Group (1 mentions)
Gelatin, by Sinopharm (1 mentions)
Dmem f12, by Meilun (1 mentions)
Trypsin, by Sangon (1 mentions)
Phosphate buffered saline (pbs), by Sangon (1 mentions)
Arpe 19, by BNCC (1 mentions)
Arpe 19, by Procell (1 mentions)
Dmem f12, by Procell (1 mentions)
D glucose, by Sangon (1 mentions)
2 protocols using antibiotic antimycotic
1
Silk Hydrogel for Chondrocyte Encapsulation
2
High-glucose-induced ARPE-19 cell model
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
We used the Human retinal pigment epithelial (ARPE-19) cell line bought from BeNa Culture Collection (Beijing, China). The ARPE-19 cells were cultured using DMEM/F12 (Procell, China) medium with 10% fetal bovine serum (Sangon Biotech, Shanghai, China) and 1% Antibiotic-Antimycotic (Sangon Biotech) at 37°C with 5% CO 2 . When the cell density maintained 80% to 90%, we washed the cells with PBS (Sangon Biotech) and passaged them with 0.05% trypsin (1:3) (Sangon Biotech). Then, the cells were cultured in the medium containing 30 mmol/L D-glucose (Sangon Biotech) to establish a high-glucose model. After culturing for 48 hours, the cell samples were collected for subsequent experiments. Kaempferol was diluted into series of concentrations (5, 10, 15, 20, 25 μM) with the basal medium.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!