Vibrotome

Manufactured by Campden Instruments

Sourced in United Kingdom

The Vibrotome is a laboratory instrument used for cutting thin, uniform sections of biological samples, such as tissues or organs. It utilizes a vibrating blade to make precise, clean cuts, enabling researchers to obtain high-quality specimens for microscopic examination and analysis.

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Lab products found in correlation

Sprague dawley rats, by Orient Bio (2 mentions) Axio imager 2, by Zeiss (1 mentions) Slowfade gold, by Thermo Fisher Scientific (1 mentions)

7 protocols using vibrotome

Muscimol-Based Neuronal Inactivation in Mice

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Mice were bilaterally implanted with guide and dummy cannulae (Plastics One) over RSP and given at least 96 h for recovery. On experiment day, the animals were infused with 1.0 – 1.2 μL Muscimol-BODIPY-TMR-X (0.5mg/ml, ThermoFisher) or vehicle per hemisphere and tested in the escape behaviour assay. Mice were anesthetised and internal cannulae projecting 0.5 mm below guide cannulae, were inserted and sealed with Kwik-Sil. Muscimol or vehicle were then infused at a rate of 150–200 nl min⁻¹ using a microinjection unit (Hamilton, 10μl syringe; in Kopf Instruments Model 5000) connected to the internal canula through tubing (Plastics One) and a plastic disposable adaptors (Plastics One). Mice were given 35 minutes to recover before starting the escape behaviour assay.
To confirm infusion site, immediately upon termination of the behavioural assay, mice were anaesthetized with isoflurane (5%, 2 l min^-1) and decapitated. The brain was sectioned coronally (100 μm) with a vibrotome (Campden Instruments) in ice-cold PBS (0.1 M), directly transferred to 4% paraformaldehyde (PFA) solution, and kept for 20 min at 4 °C. The slices were then rinsed in PBS, counter-stained with 4',6-diamidino-2-phenylindole (DAPI; 3 μM in PBS), and mounted on slides in SlowFade Gold (Thermo Fisher) before imaging (Zeiss Axio Imager 2) on the same day.

Campagner D., Vale R., Tan Y.L., Iordanidou P., Arocas O.P., Claudi F., Stempel A.V., Keshavarzi S., Petersen R.S., Margrie T.W, & Branco T. (2022). A cortico-collicular circuit for orienting to shelter during escape. Nature, 613(7942), 111-119.

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Striatal Microdialysis in Rats

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As our Animals (Scientific Procedures) Act, 1986 project licence did not permit radiotelemetry and microdialysis to be performed in the same animal, microdialysis was measured in a separate cohort of rats using an identical protocol as previously described (Rodsiri et al., 2011) . A CMA 12 polyurethane guide cannula (CMA Microdialysis AB, Kista, Sweden) was implanted above the striatum using stereotaxic coordinates anteriorposterior +0.48, medial-lateral ±3.0, dorsal-ventral -3.6 from Bregma (Paxinos and Watson, 1997) were rapidly removed and stored in 4% paraformaldehyde until sectioned (150µm coronal slices) using a vibrotome (Campden Instruments Ltd, Loughborough, UK). Location of the probe in the striatum was confirmed under a light microscope using Paxinos and Watson Rat Brain Atlas (1997) .

Shortall S.E., Spicer C.H., Ebling F.J., Green A.R., Fone K.C, & King M.V. (2016). Contribution of serotonin and dopamine to changes in core body temperature and locomotor activity in rats following repeated administration of mephedrone. Addiction biology, 21(6).

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Mouse Brain Slice Seizure-like Events

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Adult male or female C57 mice were anaesthetised with CO₂ and the brain rapidly dissected and submerged in ice-cold “normal” aCSF. The brain was sliced coronally in ice-cold normal aCSF into 400-µm-thick sections between Bregma 1 to -5 using a vibrotome (Campden Instruments Ltd., Sileby, Leics, UK). Subsequently, the slices were immersed in aCSF void of magnesium (no-Mg aCSF) at room temperature (approximately 21 °C) for at least 60 min. Exposure to no-Mg aCSF activates the tissue by unblocking NMDA receptors [28 (link)], resulting in the generation of repeating, spontaneous paroxysmal events known as seizure-like events (SLEs).

Voss L.J, & Sleigh J.W. (2020). A Metabolic Mechanism for Anaesthetic Suppression of Cortical Synaptic Function in Mouse Brain Slices—A Pilot Investigation. International Journal of Molecular Sciences, 21(13), 4703.

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PGS-co-PEG Samples Cutting Protocol

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PGS-co-PEG samples were cut into 300 μm thick discs via vibrotome (5100mz, Campden Instruments, Loughborough, UK). The vibrotome frequency, amplitude and speed were set at 80 Hz, 1.5 mm and 0.10 mm s⁻¹, respectively. The cutting was conducted in wet conditions (deionized water was added to the water bath).

Aleemardani M., Trikić M.Z., Green N.H, & Claeyssens F. (2022). Elastomeric, bioadhesive and pH-responsive amphiphilic copolymers based on direct crosslinking of poly(glycerol sebacate)-co-polyethylene glycol. Biomaterials Science, 10(24), 7015-7031.

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Preparation of Visual Cortical Slices

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Visual cortical slices were prepared from 5- (P35 to P41) and 8-week-old (P56 to P62) Sprague-Dawley rats of either sex (Orientbio Inc., Seoul, Korea), which were raised under the standard conditions (23±1℃, 12/12 hours light/dark cycle). Animal care and surgical procedures were conducted with the approval of the Institutional Animal Care and Use Committee of the School of Medicine at The Catholic University of Korea, and were consistent with the National Institutes of Health Guide for the Care and Use of Laboratory Animals. The animals were sedated with chloral hydrate (400 mg/kg, i.p.) before decapitation. The brains were quickly removed to cold dissection medium consisting of (in mM) 125 NaCl, 2.5 KCl, 1 CaCl₂, 2 MgSO₄, 1.25 NaH₂PO₄, 25 NaHCO₃, and 10 D-glucose, bubbled with carbogen (95% O₂/5% CO₂). Then coronal slices of the occipital cortex were prepared in 300 µm of thickness on a vibrotome (Campden Instruments, Leics, UK). The slices were recovered for 40 min at 37℃ in a submerging chamber with carbogenated dissection medium, and were maintained at room temperature before recording.

Joo K., Rhie D.J, & Jang H.J. (2015). Enhancement of GluN2B Subunit-Containing NMDA Receptor Underlies Serotonergic Regulation of Long-Term Potentiation after Critical Period in the Rat Visual Cortex. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 19(6), 523-531.

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Brain Slice Preparation for Electrophysiology

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The brain was rapidly dissected from adult male and female C57 mice anaesthetised with CO

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, then submerged in ice-cold ‘Normal’ artificial cerebrospinal fluid (aCSF); see Table 3 for chemical composition. Coronal 400

μ

m-thick sections were sliced between +1 to −5 Bregma using a vibrotome (Campden Instruments Ltd., Sileby, Leics, UK). The slices were then immersed at room temperature in no-magnesium (no-Mg) aCSF (see Table 3, columns 4–6). All solutions were made in double-distilled water and pre-oxygenated (95% oxygen, 5% nitrogen) using an oxygen concentrator (Perfecto2, Invacare, Auckland, New Zealand) prior to use.

Steyn-Ross D.A., Steyn-Ross M.L., Sleigh J.W, & Voss L.J. (2023). Determination of Krogh Coefficient for Oxygen Consumption Measurement from Thin Slices of Rodent Cortical Tissue Using a Fick’s Law Model of Diffusion. International Journal of Molecular Sciences, 24(7), 6450.

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Preparation of Visual Cortical Slices

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Visual cortical slices were prepared from 5-week-old Sprague-Dawley rats of either sex (Orientbio Inc., Sungnam, Korea), maintained under standard conditions (23±1℃, 12/12 h light/dark cycle). Animal care and surgical procedures were conducted under the approval of the Institutional Animal Care and Use Committee of School of Medicine at The Catholic University of Korea (approval no. 2012-0098-01). The animals were sedated with chloral hydrate (400 mg/kg, i.p.) and the brains were removed quickly to ice-cold dissection medium after the tail pinch reflex disappeared. Coronal sections of the occipital cortex (thickness, 300 µm) were prepared on a vibrotome (Campden Instruments, Leics, UK). The slices were allowed to recover in a submerging chamber for 40 min at 37℃ before being maintained at room temperature. The dissection and storage medium consisted of 125 mM NaCl, 2.5 mM KCl, 1 mM CaCl₂, 2 mM MgSO₄, 1.25 mM NaH₂PO₄, 25 mM NaHCO₃, and 10 mM D-glucose, bubbled with carbogen (95% O₂/5% CO₂). The slices were transferred to a recording chamber containing carbogenated artificial cerebrospinal fluid (ACSF, 1.5-2 ml/min) (125 mM NaCl, 2.5 mM KCl, 2 mM CaCl₂, 1 mM MgSO₄, 1.25 mM NaH₂PO₄, 25 mM NaHCO₃, and 10 mM D-glucose) at 32~33℃.

Joo K., Yoon S.H., Rhie D.J, & Jang H.J. (2014). Phasic and Tonic Inhibition are Maintained Respectively by CaMKII and PKA in the Rat Visual Cortex. The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology, 18(6), 517-524.

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