CRA-1 was purchased from Kyokuto (Tokyo, Japan). Mouse IgG and anti-human IgE fluorescein isothiocyanate (FITC) antibodies were purchased from Biosources (Burlingame, CA, USA). Anti-mouse IgG FITC antibody was purchased from Jackson ImmunoResearch Laboratories, Inc. (Baltimore, PO, USA). The RPMI-1640 medium, antibiotics, antimycotics, and fetal bovine serum (FBS) were obtained from GIBCO BRL (Gaithersburg, MD, USA). The 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)- 2-(4-sulfophenyl)-2H-tetrazolium, inner salt (MTS) was purchased from Promega (Madison, WI, USA). The TRIzol reagent, Superscript II reverse transcriptase, and oligo(dT)12–18 primer were purchased from Invitrogen (Carlsbad, CA, USA). The Taq DNA polymerase was purchased from Roche (Mannheim, Germany). The dNTP set was purchased from Amersham Pharmacia Biotech Inc. (Piscataway, NJ, USA). All other reagents, including hydroxyethyl piperazineethanesulfonic acid (HEPES), Fura 2-acetoxymethyl ester (AM), dimethylsulfoxide, histamine, and o-phthalaldehyde (OPA) were purchased from Sigma Chemicals (St. Louis, MO, USA).
Anti mouse igg fitc antibody
Manufactured by Jackson ImmunoResearch
Sourced in United States, United Kingdom
The Anti-mouse IgG FITC antibody is a secondary antibody conjugated with the fluorescent dye FITC (Fluorescein Isothiocyanate). It is designed to detect and bind to mouse immunoglobulin G (IgG) antibodies in various immunoassays and imaging applications.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Superscript 2 reverse transcriptase, by Thermo Fisher Scientific (1 mentions)
Trizol reagent, by Thermo Fisher Scientific (1 mentions)
Oligo dt 12 18 primer, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
O phthalaldehyde, by Merck Group (1 mentions)
Antimycotics, by Thermo Fisher Scientific (1 mentions)
Histamine, by Merck Group (1 mentions)
Taq dna polymerase, by Roche (1 mentions)
Hydroxyethyl piperazine ethane sulfonic acid hepes, by Merck Group (1 mentions)
3 4 5 dimethylthiazol 2 yl 5 3 carboxymethoxyphenyl 2 4 sulfophenyl 2h tetrazolium inner salt, by Promega (1 mentions)
Hoechst 33342, by Thermo Fisher Scientific (1 mentions)
Crystal violet, by Merck Group (1 mentions)
Eclipse ts100, by Nikon (1 mentions)
Anti α tubulin antibody, by Merck Group (1 mentions)
Alexa fluor 647 conjugated wheat germ agglutinin wga, by Thermo Fisher Scientific (1 mentions)
Vectashield mounting medium, by Vector Laboratories (1 mentions)
2 protocols using anti mouse igg fitc antibody
1
Evaluating Cellular Responses using MTS Assay
2
Microscopic Evaluation of Glioblastoma Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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To detect morphological changes, treated U87-MG and U87-MGshp53 cells in comparison to untreated cells were fixed with 100% methanol, stained with 0.5% crystal violet (Sigma-Aldrich, St. Louis, MO, USA), and analyzed by an inverted light microscope (Nikon eclipse TS100, Nikon, Tokio, Japan).
Treated glioblastoma cells were fixed using 4% paraformaldehyde in PBS. Subsequently, cell membranes or cytoskeleton were stained with Alexa Fluor 647 conjugated Wheat Germ Agglutinin (WGA; 1:200, Life Technologies, Carlsbad, CA, USA) or anti α-tubulin antibody (1:500, Sigma-Aldrich, St. Louis, MO, USA), respectively, followed by a secondary anti-mouse IgG-FITC antibody (1:200, Jackson ImmunoResearch, Ely, UK) according to the manufacturer’s protocols. DNA was stained using Hoechst 33342 (1:50,000, Invitrogen). The coverslips were placed upside down in a drop of Vectashield mounting medium (Vector Laboratories, Burlingame, CA, USA) on a microscope slide. The images were captured by a confocal laser scanning microscope (Leica SP5, Leica, Wetzlar, Germany) and analyzed by Fiji software (ImageJ 1.53c, National Institutes of Health, USA).
Treated glioblastoma cells were fixed using 4% paraformaldehyde in PBS. Subsequently, cell membranes or cytoskeleton were stained with Alexa Fluor 647 conjugated Wheat Germ Agglutinin (WGA; 1:200, Life Technologies, Carlsbad, CA, USA) or anti α-tubulin antibody (1:500, Sigma-Aldrich, St. Louis, MO, USA), respectively, followed by a secondary anti-mouse IgG-FITC antibody (1:200, Jackson ImmunoResearch, Ely, UK) according to the manufacturer’s protocols. DNA was stained using Hoechst 33342 (1:50,000, Invitrogen). The coverslips were placed upside down in a drop of Vectashield mounting medium (Vector Laboratories, Burlingame, CA, USA) on a microscope slide. The images were captured by a confocal laser scanning microscope (Leica SP5, Leica, Wetzlar, Germany) and analyzed by Fiji software (ImageJ 1.53c, National Institutes of Health, USA).
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