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Anti utx antibody

Manufactured by Santa Cruz Biotechnology
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The Anti-UTX antibody is a research tool used to detect and study the UTX protein, also known as KDM6A. UTX is a histone demethylase involved in the regulation of gene expression. The antibody can be used in various experimental techniques, such as Western blotting, immunoprecipitation, and immunohistochemistry, to analyze the expression and localization of the UTX protein in biological samples.

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Lab products found in correlation

Dynabeads protein g, by Santa Cruz Biotechnology (2 mentions) Western lightning plus ecl detection kit, by PerkinElmer (2 mentions) Anti rabbit igg control antibody, by Santa Cruz Biotechnology (2 mentions) Af2944, by R&D Systems (2 mentions)

2 protocols using anti utx antibody

1

Immunoprecipitation and Protein Interaction Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were incubated with 5 µg anti-UTX antibody (Bethyl), or anti-rabbit IgG control antibody (Santa Cruz) overnight at 4 °C followed by 2 h of incubation with Dynabeads Protein G for immunoprecipitation. Protein complexes bound to antibody and beads were washed 5 times and eluted with Laemmli sample buffer. Samples were resolved by SDS-PAGE. Protein interaction was analyzed by immunoblotting with anti-PLZF (R&D, AF2944), or anti-JunB primary antibodies, and goat anti-rabbit IgG-HRP secondary antibodies, followed by visualization using the Western Lightning Plus ECL detection kit (PerkinElmer).
Beyaz S., Kim J.H., Pinello L., Xifaras M.E., Hu Y., Huang J., Kerenyi M.A., Das P.P., Barnitz R.A., Herault A., Dogum R., Haining W.N., Yilmaz Ö.H., Passegue E., Yuan G.C., Orkin S.H, & Winau F. (2016). The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells. Nature immunology, 18(2), 184-195.
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2

Immunoprecipitation and Protein Interaction Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cell lysates were incubated with 5 µg anti-UTX antibody (Bethyl), or anti-rabbit IgG control antibody (Santa Cruz) overnight at 4 °C followed by 2 h of incubation with Dynabeads Protein G for immunoprecipitation. Protein complexes bound to antibody and beads were washed 5 times and eluted with Laemmli sample buffer. Samples were resolved by SDS-PAGE. Protein interaction was analyzed by immunoblotting with anti-PLZF (R&D, AF2944), or anti-JunB primary antibodies, and goat anti-rabbit IgG-HRP secondary antibodies, followed by visualization using the Western Lightning Plus ECL detection kit (PerkinElmer).
Beyaz S., Kim J.H., Pinello L., Xifaras M.E., Hu Y., Huang J., Kerenyi M.A., Das P.P., Barnitz R.A., Herault A., Dogum R., Haining W.N., Yilmaz Ö.H., Passegue E., Yuan G.C., Orkin S.H, & Winau F. (2016). The histone demethylase UTX regulates the lineage-specific epigenetic program of invariant natural killer T cells. Nature immunology, 18(2), 184-195.
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