For full length INTS15, the pcDNA3.1+/C-(k)DYK-C7orf26 plasmid was obtained from Genscript (Cat #: OHu71729D) (pcDNA3.1-INTS15-FLAG). For INTS15s, pCMV3-C7orf26-t2 was obtained from Sino Biologicals (HG27750-UT) and subsequently sub-cloned into the pFLAG-puro vector using NEBuilder Hifi DNA Assembly Master Mix (pFLAG-INTS15s-FLAG) (New England Biolabs).
pcDNA3.1-INTS15-FLAG, pFLAG-INTS15s-FLAG, and pCMV-GFP-FLAG were transfected into HEK-293T cells with Lipofectamine 2000 (Invitrogen) and selected with puromycin (Invitrogen) at 2ug/mL 24 hours after transfection. The bulk cell population was diluted to allow for the isolation of pools of cells originating from a single cell. Expression of the flag tagged INTS15, INTS15s, and GFP was confirmed by western blot.
pcDNA3.1-INTS15-FLAG, pFLAG-INTS15s-FLAG, and pCMV-GFP-FLAG were transfected into HEK-293T cells with Lipofectamine 2000 (Invitrogen) and selected with puromycin (Invitrogen) at 2ug/mL 24 hours after transfection. The bulk cell population was diluted to allow for the isolation of pools of cells originating from a single cell. Expression of the flag tagged INTS15, INTS15s, and GFP was confirmed by western blot.