Anti hif1α nb100 105

The following antibodies were used in the experiments: anti-FLAG (F3165) from Sigma-Aldrich; anti-Myc (562), anti-Strep-tag II (M211-3), and anti-hemagglutinin (HA) antibody (M180-3) from Medical & Biological Laboratories (MBL); anti-CD46 (#13241), anti-hnRNPA1 (#8443), anti-c-Myc (#9402), and anti-SRSF1 (#14902) from Cell Signaling Technology; anti-puromycin (MABE343, clone 12D10) purchased from Millipore; anti-HIF1α (NB100-105) purchased from Novus Biologicals; anti-glyceraldehyde-3-phosphate dehydrogenase (GAPDH) (sc-32233) purchased from Santa Cruz Biotechnology; goat anti-mouse immunoglobulin G (IgG) horseradish peroxidase (HRP)-linked whole antibody (31430) and goat anti-rabbit IgG HRP-linked whole antibody (31460) purchased from Thermo Scientific.

Anti-Myc (9E10), anti-His (H-15), anti-GAPDH (0411) and anti-HIF-1α (H206) antibodies were purchased from Santa Cruz. Anti-hemagglutinin (anti-HA) antibody was purchased from Covance. Anti-flag (F1804) antibody was purchased from Sigma. Anti-α-tubulin (EPR1333) and anti-Set7 (EPR5574) antibodies were purchased from Epitomics. Anti-HIF-1α (NB100–105) was purchased from Novus Biologicals. Anti-SOD2 (EPR2560Y) antibody was purchased from GeneTex. Anti-PAI-1 (612025) antibody was purchased from BD Transduction Laboratories. Anti-Histone H3 [(D1H2)XP] antibody was purchased from Cell Signaling. Anti-HIF-1α(CH₃)-K32 polyclonal antibody was obtained at Abmart with the HIF-1α-K32-me1 peptide (Shanghai, China). The active Set7 protein (14–469) was purchased from Millipore, and S-adenosylmethionine (SAM) (B903S) was purchased from New England Biolabs (NEB). 2ME2 (S1233) was purchased from Selleck, and (R)-PFI-2 was obtained from Tocris. Glucose assay kit was purchased from BioVision, and adenosine triphosphate (ATP) assay kit from Beyotime. 6-[N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) amino]-2-deoxy-D-glucose(2-NBDG), a glucose analog, was purchased from Invitrogen.