Cancer cells were treated with M-PER® Mammalian Protein Extraction Reagent (Thermo Scientific, Rockford, IL, USA), HALT Protease Inhibitor Cocktail (Thermo Scientific), and Phosphatase Inhibitor Cocktail (Thermo Scientific) to extract total protein. A total of 15–20 µg of total protein were electrophoresed per well on a SDS-polyacrylamide gel; transferred onto Immobilon-FL PVDF membranes (Millipore, Billerica, MA, USA); and further incubated with the following primary antibodies: VEGFA (sc-507), VEGFC (sc-1881), VEGFD (sc-13085), LYVE1 (sc-28190), and COX-2 (sc-1747), using antibodies (1:500 dilutions) from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Santa Cruz, CA, USA). Monoclonal GAPDH antibody (MAB374) was from Millipore, Billerica, MA, USA. After blocking with primary antibodies, overnight blots were probed with a mixture of IRDye polyclonal secondary antibodies (LI-COR Biosciences, Lincoln, NE, USA). Images were read with an Odyssey infrared imaging system (LI-COR Biosciences).
Vegfd
Manufactured by Merck Group
Sourced in United States, Switzerland
VEGFD is a laboratory equipment product developed by Merck Group. It is designed to measure and analyze vascular endothelial growth factor D (VEGF-D), a protein involved in the regulation of angiogenesis and lymphangiogenesis. The core function of VEGFD is to provide researchers and scientists with a tool to quantify and study VEGF-D levels in various biological samples.
Automatically generated - may contain errors
Lab products found in correlation
Immobilon fl pvdf membrane, by Merck Group (1 mentions)
Phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
Monoclonal gapdh antibody mab374, by Merck Group (1 mentions)
Cox 2, by Merck Group (1 mentions)
Irdye polyclonal secondary antibodies, by LI COR (1 mentions)
Odyssey infrared imaging system, by LI COR (1 mentions)
M per mammalian protein extraction reagent, by Thermo Fisher Scientific (1 mentions)
Halt protease inhibitor cocktail, by Thermo Fisher Scientific (1 mentions)
Mmp 7, by R&D Systems (1 mentions)
Guava easycyte plus, by Merck Group (1 mentions)
Mmp 2, by R&D Systems (1 mentions)
Vegf a, by Merck Group (1 mentions)
Vegfr3, by Thermo Fisher Scientific (1 mentions)
Vegfr2, by Thermo Fisher Scientific (1 mentions)
Mmp 3, by R&D Systems (1 mentions)
2 protocols using vegfd
1
Protein Extraction and Western Blot Analysis
2
Serum Biomarker Profiling Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Serum samples were obtained preoperatively or before white light cystoscopy. Venous blood (10 ml) was collected and centrifuged at 900 rpm for 10 minutes. The serum was then separated, aliquoted in 2 ml fractions and stored at −80°C until further use. Protein serum levels were assessed by the multiplexed particle-based flow cytometric cytokine assay (Vignali, 2000). Kits for VEGF-A, VEGF-C and VEGF-D were purchased from Millipore (Zug, Switzerland), for MMP-2, MMP-3 and MMP-7 from R&D Systems (Oxon, UK) and for VEGF-R2 and VEGF-R3 from eBioscience (Vienna, Austria). The procedures closely followed the manufacturer’s instructions. The analysis was conducted using a conventional flow cytometer (Guava EasyCyte Plus, Millipore, Zug, Switzerland) [22 (link)].
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