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Prisma trio

Manufactured by Siemens
Sourced in Germany

The Prisma Trio is a laboratory equipment product manufactured by Siemens. It is a high-performance imaging system designed for advanced microscopy applications. The core function of the Prisma Trio is to provide users with the ability to capture and analyze detailed images of samples at the microscopic level.

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3 protocols using prisma trio

1

Comprehensive MRI Examination Protocol

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MRI examinations were performed on a 3T MRI machine (Siemens Prisma Trio, Siemens AG, Munich, Germany) equipped with a 64-channel head coil. Each patient was scanned with a sagittal 3D FLAIR sequence (TR = 5000 ms, TE = 397 ms, TI = 1800 ms, acquired voxel size 1.0 × 1.0 × 1.0 mm) and a sagittal 3D T1-weighted sequence (TR = 2300 ms, TE = 3 ms, TI = 900 ms, flip angle = 9°, acquired voxel size 1.0 × 1.0 × 1.0 mm). All patients underwent diffusion tensor imaging and tractography (Neuro 3D, Siemens). The specific parameters were as follows: TR = 2680 ms, TE = 48 ms, FOV = 280 mm, matrix size = 128 × 128, number of signal averages = 1, slice thickness = 4.0 mm, b = 0 and 500 s/mm2, and the scanning time was 6 minutes and 49 seconds. Pre-surgical blood oxygen level-dependent functional MRI (fMRI) was performed using an echo planar imaging/gradient echo protocol (TR = 3000 ms, TE = 30 ms, slices number = 38, slice thickness = 3.5 mm, FOV = 280, matrix size = 128 × 128, voxel dimension = 3.5 × 3.5 × 3.5 mm, flip angle = 90, bandwidth = 4808 Hz/Px).
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2

Structural MRI for Cortical Implant Design

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To guide implant design and reconstruct cortical surfaces, T1-weighted sMRI images (MPRAGE, 0.5 mm isotropic) were collected from both monkeys before implant surgery under anesthesia (following the same protocol as in implant surgery except not using sevoflurane), inside a 3T MRI scanner (Prisma/Trio, Siemens for monkey J/N) using a saddle-shaped, single-loop receive coil (diameter = 10.2/12.7 cm for monkey J/N). In each monkey, four runs of T1 scans were collected and averaged to achieve a two-fold increase of signal-to-noise ratio (SNR). To validate the positioning of electrodes, in-session T1-weighted sMRI images (MPRAGE, 0.5 mm isotropic) were collected at the beginning of every EM-fMRI session and often at the end too. During both pre-implant sMRI and EM-fMRI sessions, animals were put in an MRI-compatible stereotaxis frame (David Kopf Instruments).
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3

Multimodal MRI Acquisition Protocol

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The various types of MRI images—anatomic (T1-weighted), diffusion and functional—were acquired with a 3-T Siemens Prisma Trio whole-body scanner (Siemens Medical System, Erlangen, Germany), equipped with a 32-channel head coil. The full MRI protocol is described in the Protocol (Appendix C).
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