Pcr kit

APAP, adenosine 5’-triphosphate (ATP), dimethyl sulfoxide (DMSO), dithiothreitol (DTT), and N-2-hydroxylpiperazine-N’-2-ethanesulfonic acid (HEPES) were products of Sigma Chemical Company. Morphine, tapentadol, and O-DMT were from Cayman Chemical. Cellulose thin-layer chromatography (TLC) plates were from Merck (EMD Millipore Corporation). Carrier-free sodium [³⁵S]sulfate was from American Radiolabeled Chemicals. 3’-Phosphoadenosine-5’-phospho[³⁵S]sulfate (PAP[³⁵S]) was synthesized using ATP and carrier-free [³⁵S]sulfate according to a previously established protocol [27 (link)]. X-Ray films were from Research Products International Corporation. Prime STAR^® GXL DNA Polymerase was a product of Clontech Laboratories, Inc. Protein molecular weight markers were from Bioland Scientific LLC. PCR kit was a product of G-Biosciences. QIAprep^® Spin Miniprep Kit was from QIAGEN. Ecolume scintillation cocktail was from MP Biomedical LLC. Glutathione Sepharose^TM was a product of GE Healthcare Bio-Sciences. All other chemicals were of the highest grades commercially available.

Adenosine 5′-triphosphate (ATP), dithiothreitol (DTT), dimethyl sulfoxide (DMSO), N-2-hydroxylpiperazine-N′-2-ethanesulfonic acid (HEPES), DA, EP and 5-HT were products of Sigma Chemical Company (St. Louis, MO, USA). NE was purchased from Cayman Chemical Company (Ann Arbor, MI, USA). Cellulose thin-layer chromatography (TLC) plates were from EMD Millipore Corporation (Burlington, MA, USA). 3′-Phosphoadenosine-5′-phospho[³⁵S]sulfate (PAP[³⁵S]) was prepared using ATP and carrier-free [³⁵S]sulfate based on a previously established protocol [31 (link)]. Oligonucleotide primers were synthesized by Eurofins Genomics (Louisville, KY, USA). X-Ray films were products of Research Products International Corporation (Mt Prospect, IL, USA). Prime STAR GXL DNA Polymerase was purchased from Clontech Laboratories, Inc. (Mountain View, CA, USA). PCR kit was from G Biosciences (St. Louis, MO, USA). Protein molecular weight markers were from Bioland Scientific LLC (Paramount, CA, USA). QIAprep Spin Miniprep Kit was a product of QIAGEN (Germantown, MD, USA). Ecolume scintillation cocktail was from MP Biomedical LLC (Irvine, CA, USA). Glutathione Sepharose was purchased from GE Healthcare Life Sciences (Pittsburgh, PA, USA). All other chemicals were of the highest grades commercially available.

Phenylephrine (chemical purity: 99.4%) and Ecolume scintillation cocktail were purchased from MP Biomedicals, LLC. (Irvine, CA, USA). Salbutamol (chemical purity: ≥98%) was from Cayman Chemical (Ann Arbor, MI, USA). Dimethyl sulfoxide (DMSO), adenosine 5’-triphosphate (ATP), N-2-hydroxylpiperazine-N’−2-ethanesulfonic acid (HEPES), and dithiothreitol (DTT) were products of Sigma Chemical Company (St. Louis, MO, USA). 3’-Phosphoadenosine-5’-phospho[³⁵S]sulfate (PAP[³⁵S]) was prepared using ATP and free [³⁵S]sulfate based on a previously established protocol [23 (link)]. Cellulose TLC plates were purchased from EMD Millipore Corporation (Burlington, MA, USA). PCR kit was from G Biosciences (St. Louis, MO, USA). Prime STAR^® GXL DNA Polymerase was a product of Clontech Laboratories, Inc. (Mountain View, CA, USA). QIAprep^® Spin Miniprep Kit was a product of QIAGEN (Germantown, MD, USA). Protein molecular weight markers were from Bioland Scientific LLC (Paramount, CA, USA). Glutathione Sepharose™ was purchased from GE Healthcare Life Sciences (Pittsburgh, PA, USA). X-Ray films were obtained from Research Products International Corporation (Mt Prospect, IL, USA). All other chemicals were of the highest grades commercially available.