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G9071

Manufactured by Promega
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The G9071 is a laboratory equipment product manufactured by Promega. It is designed to perform a specific core function, but without further details or interpretation to maintain an unbiased and factual approach.

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Lab products found in correlation

G7572, by Promega (1 mentions) G8820, by Promega (1 mentions) G9081, by Promega (1 mentions)

3 protocols using g9071

1

Quantifying NAD+ Levels in TGF-β1 Treated Cells

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Following a 48‐hour incubation in TGF‐β1, cells were trypsinized and re‐suspended in 1× PBS. The cell suspension was then mixed with a 0.2 mol/L NaOH 1% DTAB solution before the addition of 0.4 mol/L HCl, and then incubated at 60°C for 15 minutes to lyse the cells under conditions in which NAD+ is stable and NADH is degraded. NAD+ levels were then quantified using a commercially available luminescent cycling assay (G9071, Promega) as detailed in the manufacturer instructions.
Henderson J., Duffy L., Stratton R., Ford D, & O’Reilly S. (2020). Metabolic reprogramming of glycolysis and glutamine metabolism are key events in myofibroblast transition in systemic sclerosis pathogenesis. Journal of Cellular and Molecular Medicine, 24(23), 14026-14038.
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2

Oxidative Stress and Metabolic Assays

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H2O2 (ROS-Glo), ATP (CellTiter-Glo) and NAD/NADH (NAD/NADH-Glo) were assayed at the indicated time points during or after treatments using standard chemical assays following manufactures’ protocols. (Promega G8820, Promega G9071, Promega G7572).
Su X., Wang J., Jiang L., Chen Y., Lu T., Mendonca M.S, & Huang X. (2021). PCNA Inhibition Enhances the Cytotoxicity of β-Lapachone in NQO1-Positive Cancer Cells by Augmentation of Oxidative Stress-induced DNA Damage. Cancer letters, 519, 304-314.
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3

Luminescence-based NAD+/NADP+ Quantification

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The NAD+ and NADP+ measurements are based on luminescence and were purchased from Promega (G9071, G9081). The reaction mixtures were prepared according to the manufacturer’s instructions. The cells were treated in 50 µl medium in a white 96 well luminescence plate. After the treatment 50 µl assay buffer were added and the mixture was incubated for 90 min before measuring the luminescence signal.
Hörmann P., Delcambre S., Hanke J., Geffers R., Leist M, & Hiller K. (2021). Impairment of neuronal mitochondrial function by l-DOPA in the absence of oxygen-dependent auto-oxidation and oxidative cell damage. Cell Death Discovery, 7, 151.
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