Anti cd3 apc cy7 sp34 2

Manufactured by BD

Sourced in Germany

Anti-CD3-APC-Cy7 (SP34–2) is a fluorescently labeled monoclonal antibody that binds to the CD3 cell surface antigen. It can be used in flow cytometry applications to identify and characterize T cells.

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Lab products found in correlation

Facsuite software, by BD (1 mentions) Anti cd38 pe cy7, by BD (1 mentions) Facsverse flow cytometry, by BD (1 mentions) Anti cd161 apc, by BD (1 mentions) Anti cd8 percp cy5, by BD (1 mentions) Fcr blocking reagent, by Miltenyi Biotec (1 mentions) Anti cd4 fitc, by BD (1 mentions) Lysing solution, by BD (1 mentions) Anti cd4 percp l200, by BD (1 mentions) Facscanto 2, by BD (1 mentions) Anti cd3 apc cy7, by BD (1 mentions)

2 protocols using anti cd3 apc cy7 sp34 2

Multicolor Flow Cytometry Immunophenotyping

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The staining procedure was the same as described before. In short, after thawing, samples were treated with FcR Blocking Reagent (Miltenyi Biotech, Germany) and stained accordingly with human anti-bodies (mAbs). They were anti-CD8-PerCP-Cy5.5, anti-CD3-APC-Cy7 (SP34–2), anti-CD4-FITC, anti-Vα 7.2-PE, anti-CD161-APC, anti-CD38-PE-Cy7, anti-CD279-PE-Cy7 and all of them were from BD Biosciences (Heidelberg, Germany). Then samples were washed. Acquisition was carried out by six-color flow cytometry using FACSVerse™ flow cytometry (BD Biosciences) with FACSuite software (BD Biosciences). Analyses of the data were made by FlowJo software (Tree Star, Ashland, OR, USA).

Li C., Lu Z., Bi K., Wang K., Xu Y., Guo P., Chen Y., Zhou P., Wei Z., Jiang H, & Cao Y. (2019). CD4+/CD8+ mucosa-associated invariant T cells foster the development of endometriosis: a pilot study. Reproductive Biology and Endocrinology : RB&E, 17, 78.

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Multiparameter Flow Cytometry for Immune Cells

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Whole blood samples were treated with Lysing Solution (BD) and subjected to surface staining using anti-CD3 APC-Cy7 (SP34-2; BD), anti-CD4 FITC (M-T477; BD), anti-CD8 PerCP (SK1; BD), and anti-CD20 PE (2H7; BD) antibodies. Alternatively, whole blood samples from anti-CD8 antibody-treated animals were stained with anti-CD3 APC-Cy7, anti-CD4 PerCP (L200; BD), anti-CD8 FITC (DK25; FUJIFILM), and anti-CD20 PE. Stained cells were analyzed by BD FACS Canto II.

Nomura T., Yamamoto H., Nishizawa M., Hau T.T., Harada S., Ishii H., Seki S., Nakamura-Hoshi M., Okazaki M., Daigen S., Kawana-Tachikawa A., Nagata N., Iwata-Yoshikawa N., Shiwa N., Iida S., Katano H., Suzuki T., Park E.S., Maeda K., Suzaki Y., Ami Y, & Matano T. (2021). Subacute SARS-CoV-2 replication can be controlled in the absence of CD8+ T cells in cynomolgus macaques. PLoS Pathogens, 17(7), e1009668.

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