Tumors and skin samples were excised and tissues were fixed in 4% formalin and subsequently embedded in paraffin. H/E stained tissue sections were obtained using standard procedures. Histopathological examination and immunostainings were carried out as described previously.35 (link) Primary antibodies against cleaved caspase-3 (AF835) (R and D systems, Minneapolis, MN, USA), CPD (Thymine dimers, MC-068) (Kamiya biomedicals, Seattle, WA, USA), keratin 14 (PRB-155-P) (Covance, Princeton, NJ, USA), keratin 10 (PRB-159-P) (Covance) and BrdU (347580) (BD biosciences, San Jose, CA, USA) were used. Alexa 488 linked anti-rabbit (A11034) and anti-mouse (A21121) (Invitrogen, Carlsbad, CA, USA) secondary antibodies were used.
Brdu 347580
Manufactured by BD
Sourced in United States
BrdU (347580) is a synthetic nucleoside that is an analogue of the DNA base thymidine. It can be incorporated into the newly synthesized DNA of replicating cells, substituting for thymidine during the DNA synthesis phase of the cell cycle.
Automatically generated - may contain errors
Lab products found in correlation
Keratin 10 prb 159p, by Fortrea (1 mentions)
Af835, by R&D Systems (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Merck Group (1 mentions)
Cy3 conjugated smooth muscle actin, by Merck Group (1 mentions)
Gtx30647, by GeneTex (1 mentions)
Nb100 479, by Novus Biologicals (1 mentions)
Glut1, by Merck Group (1 mentions)
2 protocols using brdu 347580
1
Histopathological Analysis of Tumor and Skin
2
Histological Immunostaining Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Histological sample processing and immunostaining was performed as previously described (Menendez-Montes et al., 2016) . The primary antibodies used in this study were: HIF1α (NB100-479, Novus Biologicals; USA and GTX30647, Genetex, USA); cTnT (CT3, Developmental Studies Hybridoma Bank; USA); BrdU (347580, BD Biosciences; USA); Cy3-conjugated Smooth Muscle Actin (C6198, Sigma Aldrich; USA) and GLUT1 (Cat. No. 07-1401, Millipore, USA). FITC-WGA (W32466, Life Technologies; USA) was used to stain cell membrane. Nuclei were stained with DAPI (Millipore; USA).
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