Cells (2 × 106) were mixed with matrigel membrane (BD Biosciences) and were injected subcutaneously into the flanks of 4–6 week old athymic Balb-c nu/nu male mice (Taconic Farms, Germantown, NY, USA). Tumor length and width were evaluated every 2 days by vernier caliper, and the tumor volume was calculated by the formula π/6× (width) 2 × length. For NAC treatment, mice were fed with drinking water containing 40 mM NAC, which was changed every 3 days. Sixteen days post-injection, mice were euthanized, and solid tumors were removed and weighed. All animal experiments were performed in accordance with relevant guidelines and regulations and were approved by the University of Pennsylvania Institutional Animal Care and Use Committee (IACUC).
Balb c nu nu male mice
Manufactured by Taconic Biosciences
Sourced in United States
Balb-c nu/nu male mice are a laboratory mouse strain that lacks a functional immune system due to a genetic mutation. They are commonly used in research applications that require an immunodeficient animal model.
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Lab products found in correlation
2 protocols using balb c nu nu male mice
1
Subcutaneous Tumor Growth Assay
2
Subcutaneous Xenograft Tumor Model
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Cells (1 × 106) were mixed with matrigel membrane (BD Biosciences) and were injected subcutaneously into the flanks of 4–6-week-old athymic Balb-c nu/nu male mice (Taconic Farms, Germantown, NY, USA). Tumor length and width were evaluated by vernier caliper every 2 days, and the tumor volume was calculated using the formula π/6 × (width)2 × length. Sixteen days post injection, the mice were killed, and the tumors were isolated and weighed. All animal experiments were performed in accordance with relevant guidelines and regulations and were approved by the University of Pennsylvania Institutional Animal Care and Use Committee (IACUC).
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