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2 protocols using anti phospho stat1 tyr701 9170

1

Signaling pathway analysis protocol

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Compounds were stored under argon at –80 °C in 100% DMSO. Vehicle represents 0.1% DMSO unless otherwise specified. Sources: IFN-γ (PHC4031, Life Technologies), TGF-β1 (R&D Systems), paclitaxel (LC Laboratories), I-BET151 (Tocris), PMA (Calbiochem), and doxorubicin and puromycin (Sigma-Aldrich). Immunoblot antibodies: anti-FLAG (F1804), anti-Actin (A5060) and anti-CDK19 (HPA007053) from Sigma-Aldrich; anti-Smad2/3 (8685), anti-Smad2 pTail (3108), anti-STAT1 (9172), anti-phospho-STAT1 Tyr701 (9170) and anti-phospho-STAT1 Ser727 (9177), anti-CEBPA (2843), anti-ROCK1 (4035), anti-ROCK2 (9029), anti-CDK8 (4101), anti-caspase-3 (9662) anti-PARP (9532) and anti-CDK9 (2316) from Cell Signaling Technology (CST); anti-phospho-Smad2/3 T220/T179 (600-401-C48) from Rockland; anti-CDK12 (NB100-87012) and anti-CDK13 (NB100-68268) from Novus; and anti-CDK8 (A302-501A) and anti-Haspin (A302-241A) from Bethyl. ChIP antibodies: RNA pol II (Rpb1 N terminus, sc-899X lot B2713) from Santa Cruz; MED1 (A300-793A lot A300-793A-2), BRD4 (A301-985A lot A301-985A50-3), and CDK8 (A302-500A lot A302-500A-1) from Bethyl; and H3K4me3 (ab8580 lot 1308511), H3K27Ac (ab4729 lot GR104852-1), and H3K4me1 (ab8895 lot GR61306-1) from Abcam.
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2

Signaling pathway analysis protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols
Compounds were stored under argon at –80 °C in 100% DMSO. Vehicle represents 0.1% DMSO unless otherwise specified. Sources: IFN-γ (PHC4031, Life Technologies), TGF-β1 (R&D Systems), paclitaxel (LC Laboratories), I-BET151 (Tocris), PMA (Calbiochem), and doxorubicin and puromycin (Sigma-Aldrich). Immunoblot antibodies: anti-FLAG (F1804), anti-Actin (A5060) and anti-CDK19 (HPA007053) from Sigma-Aldrich; anti-Smad2/3 (8685), anti-Smad2 pTail (3108), anti-STAT1 (9172), anti-phospho-STAT1 Tyr701 (9170) and anti-phospho-STAT1 Ser727 (9177), anti-CEBPA (2843), anti-ROCK1 (4035), anti-ROCK2 (9029), anti-CDK8 (4101), anti-caspase-3 (9662) anti-PARP (9532) and anti-CDK9 (2316) from Cell Signaling Technology (CST); anti-phospho-Smad2/3 T220/T179 (600-401-C48) from Rockland; anti-CDK12 (NB100-87012) and anti-CDK13 (NB100-68268) from Novus; and anti-CDK8 (A302-501A) and anti-Haspin (A302-241A) from Bethyl. ChIP antibodies: RNA pol II (Rpb1 N terminus, sc-899X lot B2713) from Santa Cruz; MED1 (A300-793A lot A300-793A-2), BRD4 (A301-985A lot A301-985A50-3), and CDK8 (A302-500A lot A302-500A-1) from Bethyl; and H3K4me3 (ab8580 lot 1308511), H3K27Ac (ab4729 lot GR104852-1), and H3K4me1 (ab8895 lot GR61306-1) from Abcam.
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