Wang resin was purchased from Lanxiao Technology (Xi'an, China). Fmoc-protected amino acids and 1-Hydroxy-1H-benzotriazole were purchased from Jill Biochemical (Shanghai, China). Trimethylsilane and dimercaptoethane (EDT) were obtained from Sahn Chemical Technology (Shanghai, China). N, N-diisopropyl ethylamine was purchased from Kangmanlin Chemical Industry (Nanjing, China). Thioanisole, dithioglycol, trifluorocarboxylic acid and formic acid for mass spectrometry were purchased from Acladin Biochemical Technology (Shanghai, China). Piperidine, N, N-dimethylformamide were obtained from Haibang Trading (Nanjing, China). Dichloromethane, N-methylpyrrolidone, phenol, and bromophenol blue, and coomassie brilliant blue G-250 were purchased from Sinopharm Group Chemical Reagent (Shanghai, China). Acetonitrile and methanol (HPLC grade) were obtained from Merck (Darmstadt, Germany). Unless indicated, all other reagents were purchased from Sigma-Aldrich (St Louis, Missouri, USA). Peptides were synthesized using manual solid-phase peptide synthesis under the protection of nitrogen, and the mass of obtained peptides and target conjugates were confirmed by Waters ACQUITY UPLC Systems (Waters, Milford, Massachusetts, USA).
Bromophenol blue
Manufactured by Sinopharm
Sourced in China
Bromophenol blue is a pH indicator dye commonly used in biochemical and analytical laboratory applications. It exhibits a distinct color change across the pH range, making it useful for monitoring and visualizing pH changes in various experimental procedures.
Automatically generated - may contain errors
Lab products found in correlation
Coomassie brilliant blue g 250, by Sinopharm (2 mentions)
Glycine, by Merck Group (2 mentions)
Glacial acetic acid, by Sinopharm (2 mentions)
Trichloroacetic acid, by Sinopharm (2 mentions)
Sodium chloride (nacl), by Sinopharm (2 mentions)
Ethanol, by Sinopharm (2 mentions)
Waters acquity uplc system, by Waters Corporation (1 mentions)
Acetonitrile, by Merck Group (1 mentions)
Methanol, by Merck Group (1 mentions)
Dichloromethane, by Sinopharm (1 mentions)
Phenol, by Sinopharm (1 mentions)
N methylpyrrolidone, by Sinopharm (1 mentions)
Malachite green, by Sinopharm (1 mentions)
Bis acrylamide, by Merck Group (1 mentions)
Superdex 75 hr 10 30 column, by GE Healthcare (1 mentions)
Eriochrome black t, by Sinopharm (1 mentions)
Q sepharose, by GE Healthcare (1 mentions)
Deae sepharose, by GE Healthcare (1 mentions)
Sp sepharose, by GE Healthcare (1 mentions)
Evans blue, by Sinopharm (1 mentions)
6 protocols using bromophenol blue
1
Peptide Synthesis Using Solid-Phase Methodology
2
Chromatographic Purification of Enzymes
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ABTS, acrylamide, bisacrylamide, glycine, sodium dodecyl sulfate (SDS), and Tris-base were purchased from Sigma-Aldrich Chem. Co. (United States). DEAE-Sepharose, Q-Sepharose, SP-Sepharose, Superdex 75 HR 10/30 column, and molecular mass standards for FPLC were obtained from GE Healthcare Co. Ltd (Boston, MA, United States). The protein marker for SDS-PAGE was purchased from Biomed SciTech Co., Ltd. (Beijing, China). Synthetic dyes including active red (AR), bromophenol blue (BB), Coomassie brilliant blue G250 (CBB), Evans blue (EB), eriochrome black T (EBT), malachite green (MG), methyl orange (MO), reactive black (RB), reactive brilliant blue (RBB), reactive orange (RO), reactive red (RR), reactive turquoise blue (RTB), reactive yellow (RY), trypan blue (TB), and mediators including acetosyringone (AS), HBT, syringaldehyde (SA), 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO), vanillin (VAL), and violuric acid (VIA) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Other chemicals used were of analytical reagent grade.
3
Paeonia ostii Seed Extraction Protocol
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The peony seeds (Paeonia ostia, 10 kg) were obtained from Shandong Seed Industry Group CO., Ltd. (Jinan, China). Quillaja saponin was purchased from Shanxi Haosen Biological Technology Co., Ltd. (Xi’an, China). Protein Markers (14.4~97.4 kDa) were bought from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China) Mercaptoethanol, Bromophenol blue, Coomasil bright blue, glacial acetic acid, isoctane, isopropyl alcohol, methanol, n-butanol, thiobarbituric acid, sodium azide, trichloroacetic acid, and trichloromethane were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). All reagents were of analytical grade.
4
Detailed Protein Purification Protocol
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The reagents used in this experiment, including sodium chloride, ammonium sulfate, formaldehyde, bromophenol blue, trichloroacetic acid, disodium hydrogen phosphate, sodium dodecylsulfate (SDS), methyl red, sodium acetate, and ammonium persulfate (APS) were purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). A bicinchoninic acid (BCA) protein assay kit and a trypsin assay kit were purchased from the Nanjing Jian Cheng Institute of Bioengineering (Nanjing, China). Glucan gel G-50 and Q-agarose gel FF were purchased from Shanghai Cable Bridge Biotechnology Co., Ltd. (Shanghai, China). All chemical reagents were of analytical grade. The RSD of chemical reagents was less than 1 %.
5
Analyzing Flowering Cabbage Chemical Properties
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Fresh Chinese flowering cabbage was purchased from a local supermarket (Wuxi, China). Indicators (bromocresol green, methyl red sodium salt, bromophenol blue, chlorophenol red, bromothymol blue sodium, and bromocresol purple), sodium hydroxide (NaOH), ethanol, phenolphthalein, sodium tetraborate decahydrate, potassium hexacyanoferrate (II) trihydrate, zinc acetate dihydrate, silver nitrate solution, and qualitative filter paper were bought from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). N–(1–Naphthyl) ethylenediamine dihydrochloride, t-butylammonium hydroxide (TBAH), and p-toluene sulfonic acid (TsOH) were obtained from Macklin Biochemical Co., Ltd. (Shanghai, China). 5,5–dithiobis (2–nitrobenzoic acid; DTNB) was bought from Aladdin Biochemical Technology Co., Ltd. (Shanghai, China), and polyethylene glycol (PEG–400) was bought from Beijing InnoChem Science and Technology Co., Ltd. (Beijing, China). Man, Rogosa, and Sharpe (MRS) broth was purchased from Haibo Biotechnology Co., Ltd. (Qingdao, Shandong, China). Polyvinylidene fluoride (PVDF) membranes were purchased from Biosharp Biotechnology Co., Ltd. (Shanghai, China). All chemical reagents were of analytical grade and used as received.
6
Purification and Analysis of Paclitaxel
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Semen Armeniacae Amarum (SA) was purchased from Beijing Yanjing Chinese Medicinal Herbs Co. Ltd. (China). BCA kit was purchased from Nanjing Jiancheng Bioengineering Institute (China). High Q ion exchange gel was purchased from Bio-Red Laboratories (USA). The TSK-GEL G6000 PW column was purchased from TOSOH (Japan). β-MercaptoEthanol was purchased from Amresco Co. (USA).
Paclitaxel (99.5% purity), sodium dodecyl sulphate, glycine and Trizma base were purchased from Sigma Chemical Co. (USA). Ethanol, acetone, carbamide, hydrochloric acid, glacial acetic acid, sodium chloride, N,N,N',N'-Tetramethyl ethylene diamine, tween 20, bromophenol blue, ammonium persulphate and EDTA were purchased from Sinopharm Chemical Reagent Co., Ltd (China).
Paclitaxel (99.5% purity), sodium dodecyl sulphate, glycine and Trizma base were purchased from Sigma Chemical Co. (USA). Ethanol, acetone, carbamide, hydrochloric acid, glacial acetic acid, sodium chloride, N,N,N',N'-Tetramethyl ethylene diamine, tween 20, bromophenol blue, ammonium persulphate and EDTA were purchased from Sinopharm Chemical Reagent Co., Ltd (China).
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