TAG concentrations were determined using TAG assay kit (Randox Laboratories Company, Crumlin, UK; catalog no.: TR210). Briefly, 2 μl of the diluted samples was incubated with 200 μl of enzyme reagent at 37°C for 5 min in a 96-well plate. The plate was read by MULTISKAN G0 (Thermo Fisher Scientific, Waltham, MA) plate reader at 500 nm. Standards and blanks were used for calculation of concentrations.
Tag assay kit
Manufactured by Randox
Sourced in United Kingdom
The TAG assay kit is a laboratory equipment product designed to quantify the levels of triglycerides (TAG) in biological samples. It provides a reliable and efficient method for the measurement of this important lipid parameter.
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Lab products found in correlation
2 protocols using tag assay kit
1
Quantification of Triglyceride Levels
2
Intracellular Triglyceride Quantification
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were harvested for measurement of intracellular TG on day 10 of differentiation. Cells were scraped in a buffer containing 1% IGEPAL-630, 150 mM NaCl and 50 mM Tris HCl. Cell lysates were sonicated (total 30 seconds) to ensure complete lysis and the protein content determined as above. The samples were then heated at 95°C for 30 min, cooled and centrifuged at 13,000 x g for 10 min. The supernatant was collected for analysis on an iLAB 650 chemistry analyser using the Randox TAG assay kit. The total intracellular TG content was normalized to protein concentration.
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