Mice (C57BL/6, 6–8 wk of age) under anesthesia were intracardially perfused with 10% Formalin. After the mice were killed, the eyes were nucleated and fixed with the same solution overnight at 4°C. After removal of the cornea and lens, the eye cups were incubated with sucrose gradient solutions (10 and 20% for 3 h each; 30% for overnight) at 4°C; this was followed by three rounds of freeze–thaw and OCT (optimal cutting temperature) embedding. Frozen tissue sections of 7-μm thickness were incubated with rabbit anti-ABCF1 (Abcam, Cambridge, MA) and mouse anti-rhodopsin antibodies (Millipore, Billerica, MA), followed by Alexa Fluor 594–labeled goat anti-rabbit immunoglobulin G (IgG) and FITC-conjugated goat anti-mouse IgG antibodies. The nuclei were visualized with DAPI. The fluorescence signals were analyzed using a fluorescence microscope.
Mouse anti rhodopsin antibodies
Manufactured by Merck Group
Mouse anti-rhodopsin antibodies are a type of laboratory reagent used in research applications. These antibodies specifically recognize and bind to the rhodopsin protein, which is a light-sensitive receptor found in the retinal cells of vertebrates. The primary function of these antibodies is to facilitate the detection and study of rhodopsin in various experimental settings.
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Lab products found in correlation
Rabbit anti flag, by Merck Group (1 mentions)
Alexa fluor 488 goat anti rabbit, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 488 goat anti mouse, by Thermo Fisher Scientific (1 mentions)
Alexa fluor 568 goat anti mouse, by Thermo Fisher Scientific (1 mentions)
Cd81 clone js 81, by BD (1 mentions)
2 protocols using mouse anti rhodopsin antibodies
1
Immunofluorescence Analysis of ABCF1 and Rhodopsin in Mouse Retina
2
Antibodies for Cell Surface Protein Analysis
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Monoclonal mouse antibodies against CD9 (clone M-L13), CD63 (clone H5C6), and CD81 (clone JS-81) were obtained from BD Pharmingen. Rabbit anti-FLAG was obtained from Sigma Aldrich. Mouse anti-rhodopsin antibodies were obtained from Millipore. Rabbit anti-CD13 (APN) antibodies were obtained from Abcam. Mouse anti-CD26 (clone M-A261) was obtained from BD Biosciences. Rabbit anti-TMPRSS2 (clone EPR3681) was obtained from Abcam. Secondary antibodies were purchased from Invitrogen and include goat-anti-rabbit-AlexaFluor 488, goat-anti-mouse-AlexaFluor 488, and goat-anti-mouse-AlexaFluor 568. Donkey-anti-goat, goat-anti-mouse, and goat anti-rabbit HRP conjugated antibodies were purchased from Thermo Scientific.
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