Mouse monoclonal anti pax7

The following materials are used in the cell culture: L-leu (#L8912), lysine (Lys, #L9037), arginine (Arg, #A6969), Collagen Ⅰ (#SCR103), Basic Fibroblast Growth Factor (bFGF, #13256-029), BSA (A1933), and DMSO (#C6295), which were purchased from Sigma-Aldrich, USA. DMEM (#11995065), L-leu-deprived DMEM powder (#88425), fetal bovine serum (FBS, #10099141C), Antibiotics-antimycotic (#15240062), and Dulbecco phosphate-buffered saline (DPBS, #14190144) were purchased from Gibco, Life, Technologies, Grand Island, NY, USA. Culture dish (10 and 6 cm), Cell ware 6, 24, and 96-well plates, Matrigel and cell strainer, were purchased from Corning, NY, USA. Accutase, Cell Detachment Solution was purchased from innovative cell technologies, SD, USA. Mouse monoclonal anti-PAX7 (#sc-81648) was purchased from Santa Cruz Biotechnology, CA, USA. Donkey anti-Mouse IgG (H + L) was purchased from Invitrogen (#A10036, Invitrogen, Carlsbad, CA, USA). DAPI (#C0065), Triton X-100 (#9002-93-1), and Tween 20 (#9008-64-5) were purchased from Solarbio (Beijing, China). CCK 8 was purchased from Good Laboratory Practice Bioscience, Montclair, CA, USA.

The primary antibodies used for immunoblotting and immunofluorescence were as follows: rabbit polyclonal anti-IK (Bethyl Laboratories, A301-708A), mouse monoclonal anti-β-actin (Santa Cruz, sc-47778), mouse monoclonal anti-Pax-7 (Santa Cruz, sc-81648), mouse monoclonal anti-MyoD (Santa Cruz, sc-377460), mouse monoclonal anti-myogenin (Santa Cruz, sc-52903), rabbit polyclonal anti-MyHC (Santa Cruz, sc-20641), mouse polyclonal anti-PARP (Cell Signaling, #9542), rabbit polyclonal anti-Cleaved Caspase-3 (Asp175) (Cell Signaling, #9661), rabbit monoclonal anti-Cleaved Caspase-9 (Asp315) (Cell Signaling, #20750), anti-GFP (Cell Signaling, #2956), anti-Bungarotoxin Alexa Fluor™ 647 conjugate (Invitrogen™, #B35450), anti-fast-twitch muscle (DSHB, F310), and anti-slow-twitch muscle (DSHB, F59). The horseradish peroxidase-conjugated goat anti-mouse or anti-rabbit IgG (Fab) secondary antibodies were purchased from Enzo Life Sciences.