Notoginsenoside R1 (NGR1, molecular weight = 933.14, purity >98.6) was obtained from Shanghai Winherb Medical S&T Development (Shanghai, China). Metformin (Sino-American Shanghai Squibb Pharmaceuticals Ltd. Shanghai, China) was used as the positive control in this study. All cell culture materials, Dulbecco’s modified Eagle’s medium (DMEM), fetal bovine serum (FBS), and penicillin/streptomycin, were obtained from Gibco (Shanghai, China). Furthermore, 3-[4,5-dimethylthylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT), zinc protoporphyrin (ZnPP), and other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). The AGE determination kit was purchased from Shanghai Westang Bio-tech co., Ltd. Primary antibodies against collagen I (ab34710), HO-1 (ab13248), caspase-3 (ab4051), and caspase-9 (ab32539) were obtained from Abcam (Cambridge, UK). Primary antibodies against TGF-β1 (sc-146), Nrf2 (sc-13032), Lamin B1 (sc-6217), Bcl-2 (sc-7382), and Bax (sc-4239) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Horseradish peroxidase (HRP)-labeled goat anti-rabbit immunoglobulin G (IgG) (H + L), HRP-labeled goat anti-mouse IgG (H + L), and β-actin antibody were obtained from Beyotime Institute of Biotechnology (Shanghai, China).
Bax sc 4239
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Bax (sc-4239) is an antibody product offered by Santa Cruz Biotechnology. It is designed to detect the Bax protein, which is involved in the regulation of apoptosis, or programmed cell death. The antibody can be used for applications such as western blotting, immunoprecipitation, and immunohistochemistry.
Automatically generated - may contain errors
Lab products found in correlation
Bca protein assay, by Thermo Fisher Scientific (2 mentions)
Ab13248, by Abcam (1 mentions)
Ab32539, by Abcam (1 mentions)
Ab4051, by Abcam (1 mentions)
Ab34710, by Abcam (1 mentions)
β actin antibody, by Beyotime (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Df3727, by Affinity Biosciences (1 mentions)
S0001, by Affinity Biosciences (1 mentions)
Protein extraction kit, by Keygen Biotech (1 mentions)
Pvdf membrane, by Merck Group (1 mentions)
X ray film, by Carestream (1 mentions)
Image pro plus 6, by Media Cybernetics (1 mentions)
Ecl kit, by Merck Group (1 mentions)
2 protocols using bax sc 4239
1
Notoginsenoside R1 Inhibits Diabetic Fibrosis
2
Analyzing Protein Expression in Co-cultured HepG2 and L02 Cells
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HepG2 (3×105) and L02 (3×105) cells were seeded into dishes and co-cultured with or without SWNHs for 48 h. Then, total protein was extracted with a protein extraction kit (Nanjing KeyGen Biotech Co., Ltd., Nanjing, China). The concentration of these proteins was monitored by the BCA protein assay (Thermo Fisher Scientific, Inc.). The proteins extracted from each sample were subjected to 10% SDS-PAGE electrophoresis and then transferred to a PVDF membrane (Merck Millipore, Darmstadt, Germany). Next, the membranes were incubated with primary antibodies [including acetyl-CoA synthetase 2 (AceCS2) (DF3727, 1:200), SCNN1A (DF9199, 1:200), SIRT3 (AF5135, 1:200), VDAC1 (DF6140, 1:200) (all from Affinity Biosciences, Cincinnati, OH, USA), Cyt-C (sc-13561, 1:50), Bax (sc-4239, 1:50) (both from Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA)] and secondary antibodies (S0001, 1:5,000; Affinity Biosciences) at 4°C overnight. The blots were projected onto X-ray film (Carestream, Shanghai, China) following visualization by chemiluminescence using the ECL kit (EMD Millipore, Billerica, MA, USA). Image Pro Plus 6.0 software (Media Cybernetics) was used to measure the intensity of protein bands.
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