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Thioetheramide pc

Manufactured by Cayman Chemical

Thioetheramide-PC is a chemical reagent used in biochemical research. It functions as a phosphatidylcholine analog.

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2 protocols using thioetheramide pc

1

PLA2-Mediated Liposomal Delivery

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Thioetheramide-PC was purchased from Cayman Chemical (Ann Arbor, MI). HSPC, DSPE-PEG2000, DOTAP, 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(Lissamine rhodamine B sulfonyl) (Rhod-PE), DSPE-PEG2000-Cy7, and NBD-PC were purchased from Avanti Polar Lipids Inc. sPLA2 enzyme from Naja mossambica was purchased from Sigma-Aldrich Co. Rabbit polyclonal antibody to sPLA2-IIa, p-P65, and p-P100 antibodies were purchased from Abcam. Bovine synovial fluid was obtained from Lampire Biological Laboratories Inc. All other chemicals were used as received. All of the buffer solutions were prepared with deionized water.
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2

sPLA2 Inhibition Assay Protocol

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The assay was conducted as described above. Instead of adding cPLA2, it was replaced by sPLA2. 48 μL of 1X phospholipase A2 reaction buffer was added into each well of a 96-wells plate. This was followed by addition of 1 μL of sPLA2, human recombinant type V (10009563, Cayman Chemical) and 1 μL of 1 mM of the respective compound or thioetheramide-PC (62750, Cayman Chemical) dissolved in DMSO. The final concentration of the test compound achieved is 10 μM. The reaction was initiated by adding 50 μL of the substrate solution and incubating at room temperature for 1 h. Fluorescence was measured using a Varioskan plate reader by exciting at 485 nm, while fluorescence emission was detected at 515 nm. Positive control was performed when 1 μL of test compound was replaced by vehicle DMSO. % activity was expressed as the mean value of duplicate wells from four experiments. % activity was calculated by the following formula: %activity=fluorescenceoftestedwellbackgroundfluorescenceofpostivecontrolbackground
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