Thioetheramide pc

The assay was conducted as described above. Instead of adding cPLA₂, it was replaced by sPLA₂. 48 μL of 1X phospholipase A₂ reaction buffer was added into each well of a 96-wells plate. This was followed by addition of 1 μL of sPLA₂, human recombinant type V (10009563, Cayman Chemical) and 1 μL of 1 mM of the respective compound or thioetheramide-PC (62750, Cayman Chemical) dissolved in DMSO. The final concentration of the test compound achieved is 10 μM. The reaction was initiated by adding 50 μL of the substrate solution and incubating at room temperature for 1 h. Fluorescence was measured using a Varioskan plate reader by exciting at 485 nm, while fluorescence emission was detected at 515 nm. Positive control was performed when 1 μL of test compound was replaced by vehicle DMSO. % activity was expressed as the mean value of duplicate wells from four experiments. % activity was calculated by the following formula: $$\%activity=\frac{fluorescenceoftestedwell-background}{fluorescenceofpostivecontrol-background}$$