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Pluronic f 127 p3000mp

Manufactured by Thermo Fisher Scientific
Sourced in United States, United Kingdom

Pluronic F-127 (P3000MP) is a non-ionic, triblock copolymer of ethylene oxide and propylene oxide. It is commonly used as a surfactant, emulsifier, and dispersant in various applications.

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2 protocols using pluronic f 127 p3000mp

1

Neutrophil Chemotaxis Assay Protocol

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Hydrogen peroxide (H2O2, 31642), adenosine 5′diphosphoribose (ADPR, A0752), N-formylmethionine-leucyl-phenylalanine (fMLP, F3506), thioglycolate (70157), lipopolysaccharide (LPS, LPS25), CXCL2 (SRP4251), complement component 5a (C5a, SRP4895A), N-(p-Amylcinnamoyl)anthranilic acid (ACA, A8486), PP2 (P0042), interleukin-8 (IL-8, CXCL8 SRP3098) were purchased from Sigma-Aldrich (Sigma-Aldrich Company Ltd., Dorset, UK). Wortmannin (W1628) was purchased from Yorlab (York, UK) and EHT1864 (3872/10) was purchased from Bio-Teche (Minnesota, US). Pluronic F-127 (P3000MP) and Fura-2AM (F1221) were purchased from Thermo Fisher Scientific (Thermo Fisher Scientific Life Technologies, Waltham, Massachusetts, U.S). The RAL DIFF-QUIK kit (a modified version of the May-Grünwald-Giemsa stain) (RAL555) was purchased from RAL Diagnostics, (RAL Diagnostics, Martillac, France).
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2

Comprehensive Toolkit for Calcium Imaging and Mitochondrial Analysis

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Cal-520/AM (ab171868) and mitoTEMPO (ab144644) were purchased from Abcam (United Kingdom). Caged IP3 (cag-iso-2–145–100) was purchased from SiChem (Germany). ACh (A6625), apamin (A1289), dihydroethidium (D7008), GSK1016790A (G0798), oligomycin (O4876), ouabain (O3125), L-NA (N5501), L-NAME (N5751), MSDC-0160 (mitoglitazone, SML1884), PE (P6126), propidium iodide (P4864), rotenone (R8875), SNP (S0501), TEMPOL (4-HYDROXY-TEMPO; 176141), TRAM-34 (T6700), and UK5099 (PZ0160) were purchased from Sigma-Aldrich (United States). Pluronic F127 (P3000MP) was purchased from ThermoFisher (United Kingdom). Collagenase (Type 2; LS004176) was purchased from Worthington (United States). All other chemicals were obtained from Sigma-Aldrich.
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