SignalSilence Usp9X siRNA I #6308 was purchased from Cell Signaling. Non-targeting siRNA-pool (ON-TARGETplus Non-targeting Pool, # D-001810-10-05) and Mcl-1 (SMARTpool: ON-TARGETplus Mcl-1 siRNA, L-004501-00-0005) were purchased from Thermo Fisher Scientific (Pittsburgh, PA). MPNST cell lines were transfected as previously described33 (link). Cells were plated on 12 wells plates. After 24 hours, they have been incubated with the formed complexes of Lipofectamine 2000 (Invitrogen, Carlsbad, CA, USA) and the respective siRNA (12-well condition) in DMEM without FBS and antibiotics. The day after, FBS was added to a total concentration of 2%.
Smartpool on targetplus mcl 1 sirna
Manufactured by Thermo Fisher Scientific
SMARTpool: ON-TARGETplus Mcl-1 siRNA is a laboratory product designed to target the Mcl-1 gene. It is a pool of four individual siRNA duplexes that work together to effectively silence the Mcl-1 gene.
Automatically generated - may contain errors
Lab products found in correlation
On targetplus non targeting pool, by Thermo Fisher Scientific (3 mentions)
Lipofectamine 2000, by Thermo Fisher Scientific (3 mentions)
Mcl 1, by Thermo Fisher Scientific (2 mentions)
Bcl2 associated x (bax), by Thermo Fisher Scientific (1 mentions)
Bak sirna, by Thermo Fisher Scientific (1 mentions)
Pmaip1 sirna, by Thermo Fisher Scientific (1 mentions)
3 protocols using smartpool on targetplus mcl 1 sirna
1
Silencing Usp9X and Mcl-1 in MPNST
2
siRNA Knockdown Optimization in Cell Lines
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SignalSilence® Usp9X siRNA I #6308 was purchased from CST. Non-targeting siRNA-pool (ON-TARGETplus Non-targeting Pool, # D-001810-10-05) and Mcl-1 (SMARTpool: ON-TARGETplus Mcl-1 siRNA, L-004501-00-0005) were purchased from Thermo Fisher Scientific (Pittsburgh, PA) and transfected as previously described [43 (link), 60 (link)]. Briefly, cells were incubated for 6 h with the formed complexes of Lipofectamine® 2000 (Invitrogen, Carlsbad, CA, U.S.A.) and the respective siRNA (12-well condition) in DMEM without FBS and antibiotics. After 6 h, FBS was added to a total concentration of 1.5%.
3
Silencing USP9X, BAX, Mcl-1, PMAIP1, and BAK
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SignalSilence®Usp9X siRNA I #6308 was purchased from CST. Non-targeting siRNA-pool (ON-TARGETplus Non-targeting Pool, # D-001810-10-05), BAX (SMARTpool: ON-TARGETplus BAX siRNA, # L-003308-01) and Mcl-1 (SMARTpool: ON-TARGETplus Mcl-1 siRNA, L-004501-00-0005) were purchased from Thermo Fisher Scientific. PMAIP1 siRNA and BAK siRNA were purchased from Ambion. Transfections were performed as previously described [54 (link), 55 (link)]. Briefly, cells were incubated for 6h with the formed complexes of Lipofectamine®2000 (Invitrogen, Carlsbad, CA) and the respective siRNA (12-well condition) in DMEM without FBS and antibiotics. After 6h, FBS was added to a total concentration of 1.5%.
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