Hal 100 illuminator

Manufactured by Zeiss

Sourced in United Kingdom

The HAL 100 illuminator is a laboratory equipment product manufactured by Zeiss. It is designed to provide consistent and uniform illumination for microscopy and imaging applications. The HAL 100 illuminator utilizes a halogen lamp as the light source and includes various features to control and manage the illumination, such as brightness adjustment and power regulation.

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Lab products found in correlation

Lsm 510 meta confocal microscope, by Zeiss (1 mentions) Facscanto, by BD (1 mentions) Shamrock sr 750, by Oxford Instruments (1 mentions) α plan fluar, by Zeiss (1 mentions) Axiovert 200, by Zeiss (1 mentions)

Close spelling variants of this product

HAL 100 halogen illuminator (3 citations)

2 protocols using hal 100 illuminator

Cellular Uptake of Anti-LDL Nanoformulation

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The internalization of the scFv-anti-LDL(-)-MCMN-Zn nanoformulation by murine and human macrophages was evaluated by flow cytometry and confocal microscopy. The fluorophore Rhodamine B was chemically bound to PCL and the PCL-RhoB conjugate was incorporated into the nanocapsule structure during the first step of its synthesis using a blend PCL-RhoB/PCL (1:10, w/w). Cells were incubated with 10⁴ particles/mL scFv-anti-LDL(-)-MCMN-Zn nanoformulation (containing 6.25 μg of scFv protein/mL of nanoformulation) for 3 h at 37°C and then evaluated. For flow cytometry assays, all samples were analyzed with a FACSCanto flow cytometer (BD Biosciences, NJ, USA) and the data analysis of 10,000 events, for each experimental condition, was performed using Flow Jo software (version 9.5.1, Tree Star Inc, OR, USA). For confocal microscopy analysis, images were obtained with a Zeiss LSM 510 Meta confocal microscope with a HAL 100 illuminator (Carl Zeiss Microscopy, Cambridge, UK).

Cavalcante M.F., Adorne M.D., Turato W.M., Kemmerer M., Uchiyama M.K., Asbahr A.C., Alves A.D., Farsky S.H., Drewes C., Spatti M.C., Kazuma S.M., Boss M., Guterres S.S., Araki K., Brüne B., Namgaladze D., Pohlmann A.R, & Abdalla D.S. (2021). scFv-Anti-LDL(-)-Metal-Complex Multi-Wall Functionalized-Nanocapsules as a Promising Tool for the Prevention of Atherosclerosis Progression. Frontiers in Medicine, 8, 652137.

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Optical Characterization of AuNP-DNA Origami

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UV–vis characterization was performed using the set up described in our previous work [27 ]. Briefly an inverted optical microscope (Axiovert 200, Zeiss) in transmitted light illumination (HAL 100 illuminator, Zeiss) was coupled with a microscope with 750 mm long spectrometer (Shamrock SR-750, Andor Technology plc.). The distinguishable agarose gel band representing AuNP-DNA origami hybrid structures were cut out after the electrophoretic procedure and were placed onto clean glass coverslip then mounted on XY sample stage.
The light transmitted through the sample was collected by 100× immersion objective (NA 1.45, α Plan-FLUAR, Zeiss), directed into a spectrometer, split by a diffractive grating of 600 lines per mm, and finally analysed using TE-cooled EMCCD (Newton DU971-UVB, Andor Technology plc.). The extinction spectra obtained from highly concentrated AuNP were normalized using the extinction spectra of the same gel acquired in a position without AuNP.

Zeitler P., Tannenbaum G.S., Clifton D.K, & Steiner R.A. (1991). Ultradian oscillations in somatostatin and growth hormone-releasing hormone mRNAs in the brains of adult male rats.. Proceedings of the National Academy of Sciences of the United States of America, 88(20), 8920-8924.

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