Flow cytometry was carried out to quantify the activations of immune cells in inguinal lymph nodes and in the peripheral blood. At 3, 6, and 9 days after vaccination, inguinal lymph nodes of the immunized mice (n = 6 in each group) were collected. A single lymphocyte suspension (106 cells/ml) was prepared in 0.2% FBS 1640 (Gibco) and then stained with antibodies against CD19, CD40, CD11c, CD86, MHC I, and MHC II at 4°C for 30 min. At 7 and 14 days after vaccination, blood samples were collected from the immunized mice (n = 4 in each group). A single lymphocyte suspension (106 cells/ml) was stained with antibodies against CD19 and CD40. Data were collected using the Beckman Coulter Cytoflex S flow cytometer (United States) and analyzed by CytExpert 2.0 software (Beckman Coulter).
Fbs 1640
Manufactured by Thermo Fisher Scientific
Sourced in China
The FBS-1640 is a laboratory equipment product from Thermo Fisher Scientific. It is designed for performing basic functions in a laboratory setting. The core function of the FBS-1640 is to provide a controlled environment for various laboratory processes. No further details on the intended use or specifications of the product can be provided without the risk of bias or interpretation.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco phosphate buffered saline (dpbs), by Thermo Fisher Scientific (2 mentions)
Dnase 1, by Solarbio (2 mentions)
Collagenase 2, by Solarbio (2 mentions)
Hyaluronidase, by Solarbio (2 mentions)
Cytoflex s flow cytometer, by Beckman Coulter (1 mentions)
Cytexpert 2, by Beckman Coulter (1 mentions)
3 protocols using fbs 1640
1
Immune Cell Activation After Vaccination
2
Enzymatic Tissue Digestion Protocol
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The digestion solution was composed of collagenase II (3.5 mg/mL, Solarbio, China), DNase I (0.02 mg/mL, Solarbio, China), hyaluronidase (30 U/mL, Solarbio, China), 5% FBS-1640 (Gibco, USA), and DPBS (Gibco, USA). Samples were washed, the digestion solution was added to the tissue, and the tissue was cut and incubated at 37 °C for 1 h. The digestion solution was passed through a 40-µm cell strainer and then centrifuged at 500 x g for 5 min. The reaction was terminated by adding erythrocyte lysate for 5 min and centrifuging twice at 300 x g for 5 min.
3
Enzymatic Tissue Dissociation Protocol
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The digestion solution was composed of collagenase II (3.5 mg/mL, Solarbio, China), DNase I (0.02 mg/mL, Solarbio, China), hyaluronidase (30 U/mL, Solarbio, China), 5% FBS-1640 (Gibco, USA), and DPBS (Gibco, USA). Samples were washed, the digestion solution was added to the tissue, and the tissue was cut and incubated at 37°C for 1 h. The digestion solution was passed through a 40-µm cell strainer and then centrifuged at 500 x g for 5 min. The reaction was terminated by adding erythrocyte lysate for 5 min and centrifuging twice at 300 x g for 5 min.
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