ATP (A7699), sodium pyrophosphate (S6422), DTPA (D6518), Dluciferin (L6152), adenosine-5′-phosphosulfate sodium salt (A5508, APS), Trizma base (T1503), DTT (43817), 2,3-diaminonaphthalene (D2757), E. coli lipopolysaccharide (L3129, LPS), bradykinin acetate (B3259), calcium ionophore A23187 (C7522), EGTA (E4378) were from Sigma–Aldrich. HEPES (H9897), arginine (BP370), GTP (R0461, 100 mM aqueous solution), Coomassie Plus (Bradford) Assay Kit (23236) were from Fisher Scientific. Magnesium chloride (194698) was from MP Biomedicals. NG-monomethyl- L-arginine (80200, NMMA), NG-nitro- L-arginine methyl ester (80210, NAME), DETA NONOate (82120), 7-nitroindazole (81340) were from Cayman Chemical. Murine interferon-γ (315-05) was from PeproTech.
Murine interferon γ 315 05
Manufactured by Thermo Fisher Scientific
Sourced in United States
Murine interferon-γ (315-05) is a laboratory reagent. It is a recombinant protein derived from murine (mouse) cells. Its core function is to serve as a research tool for in vitro studies.
Automatically generated - may contain errors
Lab products found in correlation
High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions)
Deta nonoate, by Cayman Chemical (1 mentions)
Coomassie plus bradford assay kit, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Griess reagent kit, by Merck Group (1 mentions)
Genejet rna purification kit, by Thermo Fisher Scientific (1 mentions)
Raw264, by American Type Culture Collection (1 mentions)
Silica gel sio2, by Merck Group (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Mtt reagent, by Merck Group (1 mentions)
2 protocols using murine interferon γ 315 05
1
Measurement of Cellular Signaling Factors
2
Macrophage Activation Assay Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Analytical
grade solvents 95% ethanol (EtOH), methanol (MeOH), n-hexane, dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol, Dulbecco’s modified Eagle’s medium
(DMEM) (41965039, Gibco, USA), fetal bovine serum (10082139,GIBCO,
USA), penicillin/streptomycin 10,000 U/mL (15140122, Gibco, USA),
Griess reagent kit (G4410, Sigma-Aldrich, St. Louis, MO, USA) for
NO quantification, MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl
tetrazolium bromide) (CT01-5 Sigma-Aldrich), and murine interferon-γ
(315-05) (PeproTech, NJ, USA) were generously donated by the British
University in Egypt (BUE). Silica gel (SiO2) and TLC plates
were obtained from Merck (Darmstadt, Germany). Lipopolysaccharide
(O111:B4 E. coli) was purchased from Merck (Darmstadt,
Germany). The murine macrophage-like cell lines RAW 264.7 (ATCC-TIB71,
Rockville, MD, USA) were gifted by the National Research Center (NRC)
(Cairo, Egypt). GeneJET RNA Purification Kit (Thermo Fisher Scientific,
Massachusetts, USA) according to manufacturer’s protocol was
used for the RNA extraction. One μg of RNA was reverse transcribed
using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher
Scientific, Massachusetts, USA) with random primer according to the
manufacturer’s instruction.
grade solvents 95% ethanol (EtOH), methanol (MeOH), n-hexane, dichloromethane (DCM), ethyl acetate (EtOAc) and n-butanol, Dulbecco’s modified Eagle’s medium
(DMEM) (41965039, Gibco, USA), fetal bovine serum (10082139,GIBCO,
USA), penicillin/streptomycin 10,000 U/mL (15140122, Gibco, USA),
Griess reagent kit (G4410, Sigma-Aldrich, St. Louis, MO, USA) for
NO quantification, MTT reagent (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl
tetrazolium bromide) (CT01-5 Sigma-Aldrich), and murine interferon-γ
(315-05) (PeproTech, NJ, USA) were generously donated by the British
University in Egypt (BUE). Silica gel (SiO2) and TLC plates
were obtained from Merck (Darmstadt, Germany). Lipopolysaccharide
(O111:B4 E. coli) was purchased from Merck (Darmstadt,
Germany). The murine macrophage-like cell lines RAW 264.7 (ATCC-TIB71,
Rockville, MD, USA) were gifted by the National Research Center (NRC)
(Cairo, Egypt). GeneJET RNA Purification Kit (Thermo Fisher Scientific,
Massachusetts, USA) according to manufacturer’s protocol was
used for the RNA extraction. One μg of RNA was reverse transcribed
using a High-Capacity cDNA Reverse Transcription Kit (Thermo Fisher
Scientific, Massachusetts, USA) with random primer according to the
manufacturer’s instruction.
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