The GSH-Px, SOD, catalase, MDA and the total protein assay kits were obtained from Nanjing Jiancheng Bioengineering Institute and Biyuntian Institute (Nanjing, China), respectively. Stock standard solutions for sodium selenite [GBW (E) 080215] as well as VE (α-tocopherol acetate) were supplied by the National Research Center for Standard Materials and Food Detection Science Institute (Beijing, China). Real-time PCR reagents were obtained from TaKaRa (Dalian, China).
Real time pcr reagent
Manufactured by Takara Bio
Sourced in China, United States, Japan
Real-time PCR reagents are a set of chemical compounds used in the real-time polymerase chain reaction (PCR) process. The core function of these reagents is to facilitate the amplification and detection of specific DNA or RNA sequences in a sample.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (4 mentions)
Total protein assay kit, by Nanjing Jiancheng (1 mentions)
Catalase, by Nanjing Jiancheng (1 mentions)
Gsh px, by Nanjing Jiancheng (1 mentions)
Recombinant human lactoferrin, by Merck Group (1 mentions)
Irak1, by Santa Cruz Biotechnology (1 mentions)
Enhanced chemiluminescence kit, by Thermo Fisher Scientific (1 mentions)
Myd88, by Santa Cruz Biotechnology (1 mentions)
Traf6, by Santa Cruz Biotechnology (1 mentions)
Nf κb, by Santa Cruz Biotechnology (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Elisa kit, by Solarbio (1 mentions)
Lipopolysaccharides (lps), by Solarbio (1 mentions)
Cell counting kit 8 (cck8), by Solarbio (1 mentions)
Gapdh, by Sangon (1 mentions)
Tnf α, by Sangon (1 mentions)
Reverse transcription pcr kit, by Takara Bio (1 mentions)
4 protocols using real time pcr reagent
1
Antioxidant Enzymes and Lipid Peroxidation
2
Lactoferrin Modulates Lung Cancer Immune Response
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Human lung epithelial adenocarcinoma cells (A549) were bought from the cell resource center, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. We purchased RPMI-1640 medium (500 mL), fetal bovine serum (FBS, 500 mL), and penicillin (100 U/mL)-streptomycin (100 mg/L) solution (100×, 100 mL) from Gibco. Recombinant human lactoferrin (partially iron saturated), with purity greater than 90%, was purchased from Sigma-Aldrich. The miR-146a primers, as well as the related reagents, were purchased from Invitrogen. The primers of TLR4, MyD88, IRAK1, TRAF6, NFKB, TNFA, IL1B, and GAPDH were purchased from Sangon. Real-time PCR reagents (for extraction and quantification of mRNA and miRNA, respectively) were purchased from Takara. Primary antibodies against ACTB, TLR4, MyD88, IRAK1, TRAF6, NFκB, TNF-α, and IL-1β, as well as the corresponding secondary antibodies, were purchased from Santa Cruz Biotechnology. The LPS, Cell Counting Kit-8, and ELISA kits for the determination of TNF-α and IL-1β, and reagents for the western blot assay were purchased from Solarbio. The enhanced chemiluminescence kit was purchased from Thermo Fisher Scientific.
3
Targeting ILK in A549 Lung Cancer Cells
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Human lung cancer cell line A549 was purchased from the Cell Bank of Shanghai (Shanghai, People’s Republic of China). Dubecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum were purchased from Hyclone (GE Healthcare Bio-Sciences Corp., Piscataway, NJ, USA). Gemcitabine (Jianze), primer synthesis (Huada Gene, Shenzhen, People’s Republic of China), Trizol reagent (Thermo Fisher Scientific, Waltham, MA, USA); real-time PCR reagent (Takara Bio Inc., Otsu, Japan), fibronectin (FN) antibody (Sigma-Aldrich Co., St Louis, MO, USA), ILK, E-cadherin, and MRP1 antibody (Abcam, Cambridge, UK), GAPDH, goat anti-mouse IgG and goat anti-rabbit IgG (Beijing ZSGB-BIO GRIGENE, Beijing, People’s Republic of China). MTT (thiazole blue) was purchased from Beyotime Institute of Biotechnology (Haimen, Jiangsu, People’s Republic of China); lentivirus coated ILK shRNA (Henan Biotools Biological Technology Co., LTD, Henan, People’s Republic of China) was also purchased.
4
Gene Expression Analysis in Rat Sciatic Nerve
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TRIzol reagent (Thermo Fisher Scientific, USA) was utilized to extract total RNA from rat sciatic nerve tissues. Then cDNA was synthesized by reverse transcription according to the instructions of the reverse transcription PCR kit (Takara, Japan), and the synthesized cDNA was tested for concentration and purity. The real-time PCR reagent (Takara, Japan) was then utilized, and the reaction system was: 95°C for 1 min; 35 cycles of 95°C for 40 s, 58°C for 40 s, and 72°C for 45 s; and 72°C for 10 min. Quantitative analysis was carried out using the 2−ΔΔCt method. The primer sequence used is listed in Table 1 .
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