Human liver cancer cell lines including PLC/PRF/5, QGY7703, Huh7, etc., normal liver cell line Chang and mouse liver cancer cell line Hepa1-6 were all purchased from Cell Bank of the Typical Culture Preservation Committee, Chinese Academy of Sciences (Shanghai, China). PLC/PRF/5, Huh7,Hepa1-6, HepG2, SK-Hep1 and HCCLM3 cells were cultured in the DMEM medium (Invitrogen, Shanghai, China).And Chang, QGY7703, BEL7402 were cultured in RPMI1640 medium (Invitrogen, Shanghai, China). All mediums were supplemented with 10% FBS and 100U/mL penicillin–streptomycin. The following antibodies were used for Western blotting: WIP1 (sc-376257) to detect human samples from Santa Cruz (Shanghai, China); WIP1 (A6204) to detect mouse samples from ABcolonal (Wuhan, China); cleaved PARP1(#9541), cleaved-caspase3 (#9661) and beta-Actin (#4970) from Cell Signaling Technology (Shanghai, China); γH2AX (phospho-Histone H2AX (s139)) (ab81299) from Abcam (Shanghai, China); ki67 (ER1802-31) from Huabio (Hangzhou, China). WIP1 plasmid was kindly provided by Prof. Zhenyu Ju at Hangzhou Normal University. GSK2830371, Diethylnitrosamine (DEN) and TCPOBOP were purchased from Sigma-Aldrich (Shanghai, China). Olaparib (HY-10162) and Veliparib (HY-10129) were purchased from MedChemExpress (Shanghai, China). Other reagents and chemicals don’t list here are commercially available.
Olaparib hy 10162
Manufactured by MedChemExpress
Sourced in China, United States
Olaparib (HY-10162) is a poly(ADP-ribose) polymerase (PARP) inhibitor. It is a small molecule that functions by blocking the activity of PARP enzymes, which are involved in DNA repair processes.
Automatically generated - may contain errors
Lab products found in correlation
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Plc prf 5, by Thermo Fisher Scientific (1 mentions)
Qgy 7703, by Thermo Fisher Scientific (1 mentions)
Hepa1 6, by Thermo Fisher Scientific (1 mentions)
Veliparib, by MedChemExpress (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
Dmem medium, by Thermo Fisher Scientific (1 mentions)
Gsk2830371, by Merck Group (1 mentions)
Tcpobop, by Merck Group (1 mentions)
Diethylnitrosamine den, by Merck Group (1 mentions)
Gemcitabine hy 17026, by MedChemExpress (1 mentions)
Hydroxyurea h8627, by Merck Group (1 mentions)
4 6 diamidino 2 phenylindole (dapi), by Beyotime (1 mentions)
Hmgb1, by Abcam (1 mentions)
Hoechst 33342, by Beyotime (1 mentions)
β actin, by ABclonal (1 mentions)
Mda mb 231, by Thermo Fisher Scientific (1 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)
Thp 1, by Thermo Fisher Scientific (1 mentions)
Mda mb 468, by Thermo Fisher Scientific (1 mentions)
4 protocols using olaparib hy 10162
1
Liver Cancer Cell Lines and Reagents
2
Multimodal Combination Therapy for Cancer
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ML323 (HY-17543), olaparib (HY-10162), niraparib (HY-10619B), and gemcitabine (HY-17026) were purchased from MedChemExpress. Hydroxyurea (H8627) was purchased from Sigma-Aldrich. I-138 (2-(2-isopropylphenyl)-9-(4-(1-methyl-4-(trifluoromethyl)-1H-imidazol-2-yl)benzyl)-7,9-dihydro-8H-purin-8-one) was synthesized in a similar manner as described in US patent application US 2017/07145012 (20 ) and experimental details are described in Supplementary Materials and Methods.
3
Investigating LPS-Induced AKI via PARP1
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The experimental animals were purchased from the Animal Experiment Center of the Second Affiliated Hospital of Harbin Medical University and kept in a constant temperature and humidity environment. All mice had a 12-h light-dark cycle and had free access to food and water. Animal experiments were conducted in strict accordance with the National Institutes of Health guidelines and approved by the Ethics Committee of the Second Affiliated Hospital of Harbin Medical University (Approval No. SYDW 2021-087, Harbin, China).
The DLT (batch number: 20140302) was purchased from Jilin Connell Pharmaceutical Co. Ltd., China. LPS (O111: B4, L2630) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Olaparib (HY-10162) was obtained from MedChemExpress (NJ, USA). GSSG, MDA, DHE, DAPI, and Hoechst 33342 were purchased from Beyotime Biotechnology (Shanghai, China). ELISA kits for mouse TNF-α, IL-6, and IL-1β and microplate assay kits for creatinine and blood urea nitrogen were provided by Jianglai Co., Ltd. (Shanghai, China). HE and PAS staining kit were purchased from Solarbio (Beijing, China). Primary antibody sources are as follows: PARP1 (DF7198; Affinity, OH, USA), HMGB1 (ab79823; Abcam, Cambridge, UK), and β-actin (AC026; ABclonal, Wuhan, China).
The DLT (batch number: 20140302) was purchased from Jilin Connell Pharmaceutical Co. Ltd., China. LPS (O111: B4, L2630) was purchased from Sigma-Aldrich (St. Louis, MO, USA). Olaparib (HY-10162) was obtained from MedChemExpress (NJ, USA). GSSG, MDA, DHE, DAPI, and Hoechst 33342 were purchased from Beyotime Biotechnology (Shanghai, China). ELISA kits for mouse TNF-α, IL-6, and IL-1β and microplate assay kits for creatinine and blood urea nitrogen were provided by Jianglai Co., Ltd. (Shanghai, China). HE and PAS staining kit were purchased from Solarbio (Beijing, China). Primary antibody sources are as follows: PARP1 (DF7198; Affinity, OH, USA), HMGB1 (ab79823; Abcam, Cambridge, UK), and β-actin (AC026; ABclonal, Wuhan, China).
4
Cell Line Cultivation and Drug Treatment
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LUSC cell lines NCI-H520 (iCell-h238) and NCI-H1703 (iCell-h262) and breast invasive carcinoma (BRCA) cell lines MDA-MB-231 (iCell-h133), HCC1937 (iCell-h327), and MDA-MB-468 (iCell-h138) were bought from iCell Bioscience Inc. GL261 was obtained from American Type Culture Collection. Human mononuclear macrophage line (THP-1) was purchased from the Shanghai Cell Bank of the Chinese Academy of Sciences (Shanghai, China). THP-1, HCC1937, and the LUSC tumor cells were maintained in RPMI 1640 medium (RPMI; 61870036, Thermo Fisher Scientific) containing 10% FBS (FBS, 16140071, Thermo Fisher Scientific) and 1% penicillin/streptomycin (10378016, Thermo Fisher Scientific). MDA-MB-231 and MDA-MB-468 cells were cultured in L-15 medium (11415064, Thermo Fisher Scientific), and GL261 cells were cultured in DMEM (10566024, Thermo Fisher Scientific) combined with 10% FBS and 1% penicillin/streptomycin. Olaparib (HY-10162) and doxorubicin (HY-15142A) were bought from MedChemExpress (MCE). The anti–mouse PD-1 (BE0146) was bought from Bio X Cell.
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