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Pa5 28932

Manufactured by Thermo Fisher Scientific
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The PA5-28932 is a laboratory equipment product manufactured by Thermo Fisher Scientific. It is designed to perform a specific core function, but a detailed and unbiased description of its capabilities cannot be provided without the risk of extrapolation or interpretation. Therefore, the description for this product is not available.

Automatically generated - may contain errors

Lab products found in correlation

Ab125015, by Abcam (2 mentions) Ab7817, by Abcam (2 mentions) Ab34710, by Abcam (2 mentions) Ab150113, by Abcam (2 mentions) Pa5 99526, by Thermo Fisher Scientific (2 mentions) Ab150077, by Abcam (2 mentions) Ab207327, by Abcam (1 mentions) Ab83053, by Abcam (1 mentions) Ab222101, by Abcam (1 mentions) Sc 130920, by Santa Cruz Biotechnology (1 mentions) Af506, by R&D Systems (1 mentions) Ma5 32090, by Thermo Fisher Scientific (1 mentions) Mab218, by R&D Systems (1 mentions) Mab628, by R&D Systems (1 mentions) Sc 8015, by Santa Cruz Biotechnology (1 mentions)

2 protocols using pa5 28932

1

Comprehensive Protein Expression Analysis

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ACTA2 (ab7817, Abcam, 1:1000 for western blot and 1:500 for operetta assay), Albumin (ab207327, Abcam, 1:100 for IF and flow cytometry), Cleaved Caspase-3 (9664, CST, 1:1000), Caspase-3 (9662, CST, 1:1000), Collagen I (ab34710, Abcam, 1:500), phospho-ERK1/2 (4370, CST, 1:1000), ERK1/2 (4695, CST, 1:1000), GAPDH (2118, CST, 1:1000), gp130 (human, PA5-28932, Thermo Fisher, 1:100), gp130 (mouse, PA5-99526, Thermo Fisher, 1:100), gp130 (extracellular, PA5-77476, Thermo Fisher, 1:1000), IgG (11E10, Aldevron), IL6 (AF506, R&D Systems, 1:1000), IL6R (flow cytometry, ab222101, Abcam, 1:100), IL6R (human, IHC and IF, MA1-80456, Thermo Fisher, 1:100), IL6R (mouse, ab83053, Abcam, 1:100), IL11 (X203, Aldevron), IL11RA (inhibition study, X209, Aldevron), IL11RA (IHC, IF, flow cytometry, ab125015, Abcam, 1:100), IL11RA (western blot, sc-130920, Santa Cruz, 1:1000), phospho-JNK (4668, CST, 1:1000), JNK (9252, CST, 1:1000), NOX4 (MA5-32090, Invitrogen, 1:1000), phospho-STAT3 (4113, CST, 1:1000), STAT3 (4904, CST, 1:1000), mouse Alexa Fluor 488 secondary antibody (ab150113, Abcam, 1:200), mouse HRP (7076, CST, 1:2000), rabbit Alexa Fluor 488 secondary antibody (ab150077, Abcam, 1:200), rabbit HRP (7074, CST, 1:2000), rat Alexa Fluor 488 secondary antibody (ab150157, Abcam, 1:200), rat HRP (31470, Santa Cruz, 1:800).
Dong J., Viswanathan S., Adami E., Singh B.K., Chothani S.P., Ng B., Lim W.W., Zhou J., Tripathi M., Ko N.S., Shekeran S.G., Tan J., Lim S.Y., Wang M., Lio P.M., Yen P.M., Schafer S., Cook S.A, & Widjaja A.A. (2021). Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH. Nature Communications, 12, 66.
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2

Fibrosis Signaling Pathway Analysis

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BIP (3177, CST), Cleaved-Caspase 3 (9664, CST), Caspase 3 (9662, CST), CHOP (5554, CST), Collagen I (ab34710, Abcam), phospho-ERK1/2 (4370, CST), ERK1/2 (4695, CST), GAPDH (2118, CST), neutralizing anti-human gp130 (MAB628, R&D Systems), anti-gp130 (PA5-99526, Thermo Fisher), gp130 (PA5-28932, Thermo Fisher, IF), IgG (11E10, Aldevron), neutralizing anti-IL11 (X203, Aldevron), commercial anti-IL11 (MAB218, R&D Systems), neutralizing anti-IL11RA (X209, Aldevron), IL11RA (ab125015, abcam, IF), phospho-mTOR (2971, CST), mTOR (2972,CST), phospho-p70S6K (9205, CST), p70S6K (2708, CST), p-PDGFRB (3124, CST), PDGFRB (3169, CST), phospho-S6 ribosomal protein (4858, CST), S6 ribosomal protein (2217, CST), ⍺-SMA (ab7817, Abcam, Operetta), ⍺-SMA (19245, CST, WB), phospho-SMAD2 (3108, CST), SMAD2 (5339, CST), phospho-STAT3 (4113, CST), STAT3 (4904, CST), XBP-1 (sc-8015, SantaCruz), mouse Alexa Fluor 488 secondary antibody (ab150113, Abcam), mouse HRP (7076, CST), rabbit Alexa Fluor 488 secondary antibody (ab150077, Abcam), rabbit HRP (7074, CST).
Widjaja A.A., Viswanathan S., Jinrui D., Singh B.K., Tan J., Wei Ting J.G., Lamb D., Shekeran S.G., George B.L., Schafer S., Carling D., Adami E, & Cook S.A. (2021). Molecular Dissection of Pro-Fibrotic IL11 Signaling in Cardiac and Pulmonary Fibroblasts. Frontiers in Molecular Biosciences, 8, 740650.
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