One-cell embryos in prophase or prometaphase were mounted between slide and coverslip as described above. Embryos were then recorded on an inverted spinning-disk confocal microscope (Leica DMI4000B- CSU 22 Yokogawa) with a 100× immersion objective (HCX PL APO 1.4 oil) controlled by Metamorph. Images were acquired with an iXon3 897 Andor camera every 0.5 s. Spindle severing was performed using a UV laser module (λ = 355 nm) iLas2 Roper, as described in (Grill et al., 2001 (link)). For each species, the laser power was adjusted so that the cut, performed at the onset of spindle elongation, generated a rapid movement of the centrosomes (due to spindle severing), but did not arrest the cells (due to excess laser power).
Hcx pl apo 1.4 oil
Manufactured by Leica
The HCX PL APO 1.4 oil is a high-performance objective lens designed for use in advanced microscopy applications. It features a numerical aperture of 1.4 and is optimized for oil immersion, providing excellent optical performance and resolution. The lens is constructed with Leica's PL APO (Apochromatic Plan) technology, ensuring accurate color correction and flat field imaging.
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Lab products found in correlation
2 protocols using hcx pl apo 1.4 oil
1
Spindle Severing in One-Cell Embryos
2
Spindle Severing Induces Centrosome Movement
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One-cell embryos in prophase or prometaphase were mounted between slide and coverslip as described above. Embryos were then recorded on an inverted spinning disk confocal microscope (Leica DMI4000B-CSU 22 Yokogawa) with a 100X immersion objective (HCX PL APO 1.4 oil) controlled by Metamorph. Images were acquired with an iXon3 897 Andor camera every 0.5 seconds. Spindle severing was performed using a UV laser module (lambda=355 nm) iLas2 Roper, as described in (Grill 2001) (link). For each species, the laser power was adjusted so that the cut, performed at the onset of spindle elongation, generated a rapid movement of the centrosomes (due to spindle severing), but did not arrest the cells (due to excess laser power).
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