100 μl peripheral blood was collected into ethylenediaminetetraacetic acid (EDTA) tubes by cardiac puncture after CO2 euthanasia. Red cells were lysed for 2 min in ACK lysis buffer (150mM NH4Cl, 10mM KHCO3, 0.1mM EDTA, pH7.4), the leukocytes were centrifuged and washed twice in PBS and the pellet resuspended in flow cytometry (FC) buffer (PBS/2% FBS) for staining. Cells were stained for 45 min on ice in FC buffer containing unlabelled CD32 (BD Biosciences, Sydney, Australia) to block Fc receptor binding and HIS48-FitC, CD11B/C-BV510, CD45R-PE (BD Biosciences), CD43-AF647, CD4-APC/Cy7 (Biolegend, San Diego, CA, USA) and CD172A-AF405 (Novus, Noble Park North, Australia). Cells were washed twice and resuspended in FC buffer containing 7AAD (LifeTechnologies, Musgrave, Australia) for acquisition on a Cytoflex flow cytometer (Beckman Coulter Life Sciences, Sydney, Australia). Single colour controls were used for compensation and fluorescence-minus-one controls were used to confirm gating. Data were analysed using FlowJo 10 (Tree Star; https://www.flowjo.com ).
Cd45r pe
Manufactured by BD
Sourced in Australia
CD45R-PE is a fluorescently-labeled monoclonal antibody that binds to the CD45R antigen expressed on the surface of certain immune cells. It is a tool used in flow cytometry applications to identify and analyze specific cell populations.
Automatically generated - may contain errors
Lab products found in correlation
Cd4 apc cy7, by BioLegend (2 mentions)
7 aad, by Thermo Fisher Scientific (2 mentions)
Cd11b c bv510, by BD (2 mentions)
Cd43 af647, by BioLegend (2 mentions)
Cd172a af405, by Novus Biologicals (2 mentions)
Cytoflex flow cytometer, by Beckman Coulter (2 mentions)
His48 fitc, by BD (2 mentions)
Cd3 fitc, by BD (1 mentions)
Cd11b apc cy7, by BD (1 mentions)
Cd62l pe, by BD (1 mentions)
Cd4 apc cy7, by BD (1 mentions)
Cxcr4 pe, by BD (1 mentions)
Ly6c pe, by BD (1 mentions)
Cd8 apc, by BD (1 mentions)
Cd11c fitc, by BD (1 mentions)
Cd11b apc, by BD (1 mentions)
7 aad, by BD (1 mentions)
Gr1 apc cy7, by BD (1 mentions)
Cyan adp flow cytometer, by Beckman Coulter (1 mentions)
Cd45 pb, by BD (1 mentions)
3 protocols using cd45r pe
1
Multicolor Flow Cytometry of Mouse Immune Cells
2
Multiparameter Flow Cytometry Analysis
3
Multifaceted Immunophenotyping of Mouse Blood
Check if the same lab product or an alternative is used in the 5 most similar protocols
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100 ul peripheral blood was collected into ethylenediaminetetraacetic acid (EDTA) tubes by cardiac puncture after CO 2 euthanasia. Red cells were lysed for 2 min in ACK lysis buffer (150mM NH 4 Cl, 10mM KHCO 3 , 0.1mM EDTA, pH7.4), the leukocytes were centrifuged and washed twice in PBS and the pellet resuspended in flow cytometry (FC) buffer (PBS/2%FBS) for staining. Cells were stained for 45 min on ice in FC buffer containing unlabelled CD32 (BD Biosciences, Sydney, Australia) to block Fc receptor binding and HIS48-FitC, CD11B/C-BV510, CD45R-PE (BD Biosciences), CD43-AF647, CD4-APC/Cy7 (Biolegend, San Diego, CA, USA) and CD172A-AF405 (Novus, Noble Park North, Australia). Cells were washed twice and resuspended in FC buffer containing 7AAD (LifeTechnologies, Musgrave, Australia) for acquisition on a Cytoflex flow cytometer (Beckman Coulter Life Sciences, Sydney, Australia). Single colour controls were used for compensation and fluorescence-minus-one controls were used to confirm gating. Data were analysed using FlowJo 10 (Tree Star; https://www.flowjo.com).
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