Elisa max deluxe set human il 1β kit

The supernatants of the macrophage cultures infected with strains Mtb H37Rv, susceptible III, and MDR III were collected after 24 h of treatment with the antibiotics and stored at −80°C for posterior evaluation of IL-1β secretion by Sandwich Enzyme-Linked Immunosorbent Assay (ELISA). In addition, the supernatants retrieved after 24 h of infection with Mtb H37Rv pre-treated with selected antibiotic concentrations were also tested. The supernatants collected from uninfected macrophages, either untreated or incubated with antibiotics or lipopolysaccharide (LPS; Sigma-Aldrich) at 100 ng/mL for 24 h, were added as controls. Quantification was conducted using ELISA Max Deluxe Set Human IL-1β kit (Biolegend) according to the instructions of the manufacturer. Absorbance was measured at 570and 450 nm in a Varioskan LUX multimode microplate reader (Thermo Scientific).

IL-1β from cell-free supernatants were quantified using ELISA MAX™ Deluxe Set Human IL-1β kit (Biolegend), following the manufacturer’s instruction. Absorbance was measured at 450 nm using a FLUOstar Optima microplate reader (BMG Labtech).