Rabbit anti pin1 antibody

After dewaxing, hydration, and antigen retrieval, paraffin sections of hippocampal tissues were blocked with 0.1% BSA (bovine serum albumin) in PBS, followed by incubation with primary antibody (rabbit anti‐Pin1 antibody, 1:200, Proteintech, 10495‐1‐AP) overnight at 4°C. Following washing three times with PBS, the sections were incubated with biotin‐labeled goat anti‐rabbit secondary antibody at room temperature for 60 min, and the nuclei were stained with DAPI (4',6‐diamidino‐2‐phenylindole). Images were captured under a fluorescence microscope.

Dulbecco’s Modified Eagle Medium (DMEM) and Roswell Park Memorial Institute (RPMI) 1640 were purchased from KeyGEN BioTECH (Nanjing, China). Fetal bovine serum (FBS) was obtained from GIBCO (New York, NY, USA). Juglone (a inhibitor of Pin1), DMSO, and oil red O were purchased from Sigma-Aldrich (St. Louis, MO, USA). BAY11-7082 and oxLDL were obtained from Meilunbio (Dalian, China). Calcein-AM was purchased from YEASEN (Shanghai, China). The primary antibodies against VCAM-1 and F4/80 were obtained from Abcam (Cambridge, UK). Rabbit antibodies against MCP-1, ERK1/2, p-ERK1/2, JNK, p-JNK, p65, and p-p65 were purchased from Cell Signaling Technology (Beverly, MA, USA). Rabbit anti-Pin1 antibody was obtained from Proteintech (Chicago, IL, USA). HRP-conjugated antibody against β-actin was purchased from Kangchen Biotechnology (Shanghai, China).