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Erk1 c16

Manufactured by Santa Cruz Biotechnology

ERK1 (C-16) is a protein-specific antibody that recognizes extracellular signal-regulated kinase 1 (ERK1). ERK1 is a member of the mitogen-activated protein kinase (MAPK) family and plays a role in the regulation of cell growth and differentiation.

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2 protocols using erk1 c16

1

SDS-PAGE Immunoblotting for Protein Quantification

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Extracts were run on an 8% to 12% SDS-PAGE gel, transferred to Immobilon-P transfer membranes (Milipore Corp.), and visualized with antibodies specific for phospho-STAT3 (Tyr705 and 3E2; Cell Signaling), STAT3 (79D7; Cell Signaling), phospho-ERK1/2 (Thr202 and Tyr204; Cell Signaling Technology), ERK1 (C16; Santa Cruz), mouse monoclonal UL99 (pp28) and HA (HA-7; Sigma), rabbit UL7, and actin (C4; Millipore).
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2

Western Blot Analysis of Cellular Signaling

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Extracts were run on 8 to 12% sodium dodecyl sulfate (SDS)-polyacrylamide gels, transferred to Immobilon-P transfer membranes (Millipore Corp.), and visualized with antibodies specific for phospho-ERK1/2 (Thr202/Tyr204; Cell Signaling Technology), ERK1 (C16; Santa Cruz), phospho-Akt (Thr308; Cell Signaling Technology), Akt (C73H10; Cell Signaling Technology), phospho-Flt3 (Tyr591; Cell Signaling Technology), FLT3 (8F2; Cell Signaling Technology), and actin (C4; Millipore).
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