Liposomes were prepared by the thin-film hydration method, as reported3 (link). In brief, the lipid formulation [DSPC (Avanti Polar Lipids, Alabaster, AL, USA), DLPC (Avanti Polar Lipids, Alabaster, AL, USA), DSPG (Genzyme, Cambridge, MA, USA), and cholesterol (Sigma, St. Louis, MO, USA) at molar ratio 3:3:2:3], along with the indicated amount of the sonosensitizer PPIX, was dissolved in a solution of chloroform:methanol 9:1. The solvent was evaporated under reduced pressure, and the lipid was redissolved in t-butanol, followed by freeze-drying. The lipid cake was hydrated with PBS, TTX solution (0.375 mg/mL PBS; Abcam, Cambridge, MA, USA), or sulforhodamine B solution (10 mg/mL PBS; Aldrich, St. Louis, MO, USA). After 10 freeze–thaw cycles, the solution was dialyzed against PBS for 48 h in a dialysis tube with a molecular mass cut-off of 1000 kDa. The dialysis media were changed with fresh PBS at least twice a day. Lipo-DMED was made following the same procedure, but with PPIX not included in the formulation and with hydration in 1 mg/mL of DMED in PBS solution.
Ttx solution
Manufactured by Abcam
Sourced in United States
TTX solution is a potent neurotoxin derived from the puffer fish. It functions by blocking sodium channels, inhibiting the propagation of action potentials in nerve and muscle cells.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using ttx solution
1
Liposome Encapsulation of Sonosensitizer PPIX
2
Liposome Encapsulation of Sonosensitizer PPIX
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Liposomes were prepared by the thin-film hydration method, as reported3 (link). In brief, the lipid formulation [DSPC (Avanti Polar Lipids, Alabaster, AL, USA), DLPC (Avanti Polar Lipids, Alabaster, AL, USA), DSPG (Genzyme, Cambridge, MA, USA), and cholesterol (Sigma, St. Louis, MO, USA) at molar ratio 3:3:2:3], along with the indicated amount of the sonosensitizer PPIX, was dissolved in a solution of chloroform:methanol 9:1. The solvent was evaporated under reduced pressure, and the lipid was redissolved in t-butanol, followed by freeze-drying. The lipid cake was hydrated with PBS, TTX solution (0.375 mg/mL PBS; Abcam, Cambridge, MA, USA), or sulforhodamine B solution (10 mg/mL PBS; Aldrich, St. Louis, MO, USA). After 10 freeze–thaw cycles, the solution was dialyzed against PBS for 48 h in a dialysis tube with a molecular mass cut-off of 1000 kDa. The dialysis media were changed with fresh PBS at least twice a day. Lipo-DMED was made following the same procedure, but with PPIX not included in the formulation and with hydration in 1 mg/mL of DMED in PBS solution.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!