The CPN ligation model of neuropathic pain was generated as described previously [18 (link), 19 (link)]. Briefly, mice were anesthetized with isoflurane (1–3%, as needed). The left CPN between the anterior and posterior muscle groups was slowly ligated with chromic gut suture 5-0 (Ethicon, Blue Ash, USA) until the appearance of twitching of the digits. The skin was sutured using 5-0 silk and cleaned with povidone iodine. Sham surgery was conducted in the same manner but the nerve was not ligated. Animals were kept in a normal cage after surgery. The mice were used for behavioral tests on postsurgical days 3–14.
Chromic gut suture 5 0
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Chromic gut suture 5-0 is a sterile, absorbable surgical suture material made from purified and processed collagen derived from the submucosal layer of the small intestine of healthy sheep. It is designed for general soft tissue approximation and/or ligation, including use in ophthalmic procedures.
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4 protocols using chromic gut suture 5 0
1
CPN Ligation Model for Neuropathic Pain
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Neuropathic and Visceral Pain Models in Mice
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A model of neuropathic pain was induced by the ligation of the CPN as described previously.12 (link) Briefly, the mice were anesthetized by intraperitoneal injection of 1% sodium pentobarbital (60 mg/kg). The CPN was visible between the anterior and posterior groups of muscles running almost transversely. The left CPN was slowly ligated with a chromic gut suture 5–0 (Ethicon) until dorsiflexor contraction of the foot was observed as twitching of the digits. Control mice were treated with sham surgery. The mechanical allodynia was tested on postsurgical day 1, 3, 5, and 7. The mice were used for analysis of cytokine protein expression.
Visceral pain was induced by the administration of zymosan as described previously.29 A volume of 0.1 ml zymosan suspension (30 mg/ml in saline; derived from Saccharomyces cerevisiae; Sigma, St. Louis, MO) was administered rectally via a 22-gauge, 24-mm-long stainless steel feeding needle into the colons of mice over a period of 2 min. Control mice were rectally administered with 0.1 ml saline. zymosan or saline was given daily for three consecutive days.
Visceral pain was induced by the administration of zymosan as described previously.29 A volume of 0.1 ml zymosan suspension (30 mg/ml in saline; derived from Saccharomyces cerevisiae; Sigma, St. Louis, MO) was administered rectally via a 22-gauge, 24-mm-long stainless steel feeding needle into the colons of mice over a period of 2 min. Control mice were rectally administered with 0.1 ml saline. zymosan or saline was given daily for three consecutive days.
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Common Peroneal Nerve Ligation for Neuropathic Pain
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The common peroneal nerve (CPN) ligation model of neuropathic pain has been used39 (link). Briefly, mice were anesthetized with sodium pentobarbital (1% wt/vol). The left common peroneal nerve between the anterior and posterior groups of muscles was ligated slowly with 5-0 chromic gut suture (Ethicon) until twitching of the digits was observed. The skin was sutured with 5-0 silk suture and cleaned with Povidone-iodine. Sham surgery was conducted in the same manner, but the nerve was not ligated. All mice were kept in a normal living cage after surgery.
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Common Peroneal Nerve Ligation Mouse Model
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The common peroneal nerve (CPN) ligation mouse model of neuropathic pain has been described previously (Vadakkan et al., 2005 (link); Li et al., 2010 (link)). Briefly, mice were anesthetized using isoflurane (1%–3%, as needed). The left CPN between the anterior and posterior groups of muscles was ligated slowly with 5–0 chromic gut suture (Ethicon), until twitching of the digits was observed. The skin was sutured using 5–0 silk suture and cleaned with povidone iodine. Sham surgery was conducted in the same manner, but the nerve was not ligated. All animals were kept in a normal living chamber post-surgery.
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